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Literature summary for 1.11.1.13 extracted from

  • Sakamoto, Y.; Nakade, K.; Nagai, M.; Uchimiya, H.; Sato, T.
    Cloning of Lentinula edodes lemnp2, a manganese peroxidase that is secreted abundantly in sawdust medium (2009), Mycoscience, 50, 116-122.
    View publication on PubMed

Application

Application Comment Organism
degradation lingnin-degrading enzymes possess oxidative activity against phenolic compoundss, which can be used for bioremediation, biobleaching, and biofuel production Lentinula edodes

Cloned(Commentary)

Cloned (Comment) Organism
lemnp2 is cloned by 3'-RACE using cDNA that is synthesized from mycelial RNA Lentinula edodes

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secreted into the medium Lentinula edodes
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Manganese
-
Lentinula edodes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
theoretical Lentinula edodes
44800
-
determined by SDS-PAGE Lentinula edodes
48000
-
determined by gel filtration Lentinula edodes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Mn2+ + H+ + H2O2 Lentinula edodes
-
Mn3+ + H2O
-
?
Mn2+ + H+ + H2O2 Lentinula edodes SR-1
-
Mn3+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Lentinula edodes B5U990
-
-
Lentinula edodes SR-1 B5U990
-
-

Purification (Commentary)

Purification (Comment) Organism
using a Toyopearl Butyl-650M, a Toyopearl DEAE-650 and a Superdex 75 HR 10/30 column Lentinula edodes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Mn2+ + guaiacol + H2O2 activity assay Lentinula edodes ?
-
?
Mn2+ + guaiacol + H2O2 activity assay Lentinula edodes SR-1 ?
-
?
Mn2+ + H+ + H2O2
-
Lentinula edodes Mn3+ + H2O
-
?
Mn2+ + H+ + H2O2
-
Lentinula edodes SR-1 Mn3+ + H2O
-
?

Subunits

Subunits Comment Organism
monomer
-
Lentinula edodes

Synonyms

Synonyms Comment Organism
LeMnP2
-
Lentinula edodes
manganese peroxidase
-
Lentinula edodes
MnP
-
Lentinula edodes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
activity assay Lentinula edodes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
activity assay Lentinula edodes

pI Value

Organism Comment pI Value Maximum pI Value
Lentinula edodes theoretical
-
3.98