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Literature summary for 1.11.1.13 extracted from

  • Giardina, P.; Palmieri, G.; Fontanella, B.; Rivieccio, V; Sannia, G.
    Manganese peroxidase isoenzymes produced by Pleurotus ostreatus grown on wood sadwust (2000), Arch. Biochem. Biophys., 376, 171-179.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning and sequencing of the gene encoding MnP2, promoter region is analyzed, nucleotide sequence of the cDNA encoding MnP1, mnp1 is located on chromosome IV, mnp2 on chromosome VI and mnp3 on chromosome V Pleurotus ostreatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
Mn2+ MnP3 Pleurotus ostreatus
0.0293 0.0294 H2O2 MnP2 and MnP3 Pleurotus ostreatus
0.0838
-
Mn2+ MnP2 Pleurotus ostreatus
0.092
-
2,6-dimethoxyphenol MnP3 Pleurotus ostreatus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Pleurotus ostreatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
1 * 41000, MnP2, 1 * 38000, MnP3, SDS-PAGE Pleurotus ostreatus
41000
-
MnP2, gel filtration Pleurotus ostreatus
47000
-
MnP3, gel filtration Pleurotus ostreatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Mn2+ + H+ + H2O2 Pleurotus ostreatus involved in lignin-degradation Mn3+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pleurotus ostreatus
-
white rot basidomycete, 3 isoenzymes: MnP1, MnP2 and MnP3
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein presence of high-mannose-type glucans Pleurotus ostreatus

Purification (Commentary)

Purification (Comment) Organism
MnP2 and MnP3, purified from poplar culture Pleurotus ostreatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Pleurotus ostreatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dimethoxyphenol + H2O2
-
Pleurotus ostreatus ?
-
?
Mn2+ + H+ + H2O2
-
Pleurotus ostreatus Mn3+ + H2O Mn3+ oxidizes 2,6-dimethoxyphenol ?
Mn2+ + H+ + H2O2
-
Pleurotus ostreatus Mn3+ + H2O Mn3+ oxidizes syringaldazine ?
Mn2+ + H+ + H2O2
-
Pleurotus ostreatus Mn3+ + H2O Mn3+ oxidizes guaiacol ?
Mn2+ + H+ + H2O2 involved in lignin-degradation Pleurotus ostreatus Mn3+ + H2O
-
?
additional information enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Pleurotus ostreatus ?
-
?
additional information no activity with veratryl alcohol Pleurotus ostreatus ?
-
?
additional information Mn2+-dependent and Mn2+-independent peroxidase activities when tested on the phenolic substrates 2,6-dimethoxyphenol, 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate, guaiacol and syringaldazine, more rapid oxidation in presence of Mn2+ Pleurotus ostreatus ?
-
?
additional information enzyme oxidizes 2,6-dimethoxyphenol Pleurotus ostreatus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 41000, MnP2, 1 * 38000, MnP3, SDS-PAGE Pleurotus ostreatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pleurotus ostreatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
100
-
Mn2+ MnP2 Pleurotus ostreatus
100
-
H2O2 MnP2 Pleurotus ostreatus
200
-
2,6-dimethoxyphenol MnP3 Pleurotus ostreatus
230
-
Mn2+ MnP3 Pleurotus ostreatus
230
-
H2O2 MnP3 Pleurotus ostreatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
MnP2: pI 3.7, MnP3: pI 3.5 Pleurotus ostreatus
2.5
-
oxidation of 2,6-dimethoxyphenol in absence of Mn2+ Pleurotus ostreatus
4
-
oxidation of Mn2+ Pleurotus ostreatus
4.5
-
oxidation of 2,6-dimethoxyphenol in presence of Mn2+ Pleurotus ostreatus

Cofactor

Cofactor Comment Organism Structure
heme iron protoporphyrin IX prosthetic group Pleurotus ostreatus