Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | slightly activates, chelates Mn3+ | Phanerodontia chrysosporium | |
alpha-hydroxy acid | - |
Phanerodontia chrysosporium | |
cellobionate | activates, chelates Mn3+ | Phanerodontia chrysosporium | |
citrate | activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential | Phanerodontia chrysosporium | |
H2O2 | H2O2-dependent | Phanerodontia chrysosporium | |
L-malate | activates by chelating and stabilizing Mn3+ | Phanerodontia chrysosporium | |
L-Tartrate | activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential | Phanerodontia chrysosporium | |
Lactate | activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential | Phanerodontia chrysosporium | |
malonate | activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential, most effective physiological chelator excreted by the fungus | Phanerodontia chrysosporium | |
additional information | dramatic stimulation by chelating organic acids as C2- and C3-dicarboxylic or alpha-hydroxyl acids facilitate the dissociation of Mn(III) from manganese-enzyme complex, greater activation with weakly binding chelators with a low binding constant, e.g. lactate or tartrate | Phanerodontia chrysosporium | |
additional information | succinate is no Mn3+ chelator and activator | Phanerodontia chrysosporium | |
nitrilotriacetate | slightly activates, chelates Mn3+ | Phanerodontia chrysosporium | |
oxalate | activates by chelating and stabilizing Mn3+ | Phanerodontia chrysosporium | |
Polyglutamate | slightly activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential | Phanerodontia chrysosporium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inhibits competitive Mn(III)-malonate formation | Phanerodontia chrysosporium | |
cellobionate | inhibits competitive Mn(III)-malonate formation | Phanerodontia chrysosporium | |
additional information | not inhibited by succinate | Phanerodontia chrysosporium | |
nitrilotriacetate | inhibits competitive Mn(III)-malonate formation | Phanerodontia chrysosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Phanerodontia chrysosporium | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
- |
Phanerodontia chrysosporium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | important component of lignin degradation system | Mn3+ + H2O | Mn3+ is stabilized by chelating agents, malonate is the most effective physiological chelator excreted by the fungus | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
- |
- |
Phanerodontia chrysosporium | - |
MnP exists as several closely related isoenzymes | - |
Phanerodontia chrysosporium | - |
white rot basidomycete | - |
Phanerodontia chrysosporium OGC101 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Phanerodontia chrysosporium |
Purification (Comment) | Organism |
---|---|
isoenzyme 1 | Phanerodontia chrysosporium |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 Mn(II) + 2 H+ + H2O2 = 2 Mn(III) + 2 H2O | ping-pong mechanism | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | each catalytic cycle step is irreversible | Phanerodontia chrysosporium | Mn3+ + H2O | alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme | ir | |
Mn2+ + H+ + H2O2 | each catalytic cycle step is irreversible | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes phenolic lignin model compounds | ir | |
Mn2+ + H+ + H2O2 | each catalytic cycle step is irreversible | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes vanillyl alcohol | ir | |
Mn2+ + H+ + H2O2 | each catalytic cycle step is irreversible | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes lignin | ir | |
Mn2+ + H+ + H2O2 | each catalytic cycle step is irreversible | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+-organic acid complexes oxidize terminal phenolic substrates in a second-order reaction | ir | |
Mn2+ + H+ + H2O2 | each catalytic cycle step is irreversible | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes thiols | ir | |
Mn2+ + H+ + H2O2 | each catalytic cycle step is irreversible | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ acts as obligatory redox coupler, oxidizing various phenols, dyes and amines | ir | |
Mn2+ + H+ + H2O2 | each catalytic cycle step is irreversible | Phanerodontia chrysosporium | Mn3+ + H2O | the diffusible product is Mn3+ | ir | |
Mn2+ + H+ + H2O2 | each catalytic cycle step is irreversible | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes amines | ir | |
Mn2+ + H+ + H2O2 | each catalytic cycle step is irreversible | Phanerodontia chrysosporium | Mn3+ + H2O | chelation of Mn3+ by organic acids stabilizes Mn3+ at a high redox potential | ir | |
Mn2+ + H+ + H2O2 | free divalent Mn is the substrate, not Mn2+-complexes | Phanerodontia chrysosporium | Mn3+ + H2O | alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme | ir | |
Mn2+ + H+ + H2O2 | free divalent Mn is the substrate, not Mn2+-complexes | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes phenolic lignin model compounds | ir | |
Mn2+ + H+ + H2O2 | free divalent Mn is the substrate, not Mn2+-complexes | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes vanillyl alcohol | ir | |
Mn2+ + H+ + H2O2 | free divalent Mn is the substrate, not Mn2+-complexes | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes lignin | ir | |
Mn2+ + H+ + H2O2 | free divalent Mn is the substrate, not Mn2+-complexes | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+-organic acid complexes oxidize terminal phenolic substrates in a second-order reaction | ir | |
Mn2+ + H+ + H2O2 | free divalent Mn is the substrate, not Mn2+-complexes | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes thiols | ir | |
Mn2+ + H+ + H2O2 | free divalent Mn is the substrate, not Mn2+-complexes | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ acts as obligatory redox coupler, oxidizing various phenols, dyes and amines | ir | |
Mn2+ + H+ + H2O2 | free divalent Mn is the substrate, not Mn2+-complexes | Phanerodontia chrysosporium | Mn3+ + H2O | the diffusible product is Mn3+ | ir | |
Mn2+ + H+ + H2O2 | free divalent Mn is the substrate, not Mn2+-complexes | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes amines | ir | |
Mn2+ + H+ + H2O2 | free divalent Mn is the substrate, not Mn2+-complexes | Phanerodontia chrysosporium | Mn3+ + H2O | chelation of Mn3+ by organic acids stabilizes Mn3+ at a high redox potential | ir | |
Mn2+ + H+ + H2O2 | important component of lignin degradation system | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ is stabilized by chelating agents, malonate is the most effective physiological chelator excreted by the fungus | ? | |
Mn2+ + H+ + H2O2 | each catalytic cycle step is irreversible | Phanerodontia chrysosporium OGC101 | Mn3+ + H2O | alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme | ir | |
Mn2+ + H+ + H2O2 | each catalytic cycle step is irreversible | Phanerodontia chrysosporium OGC101 | Mn3+ + H2O | Mn3+ oxidizes phenolic lignin model compounds | ir | |
additional information | catalytic cycle with oxidized intermediates MnP compound I and II | Phanerodontia chrysosporium | ? | - |
? | |
additional information | large substrates have no ready access to the catalytic center | Phanerodontia chrysosporium | ? | - |
? | |
additional information | enzyme oxidizes 2,6-dimethoxyphenol | Phanerodontia chrysosporium | ? | - |
? | |
additional information | catalytic cycle with oxidized intermediates MnP compound I and II | Phanerodontia chrysosporium OGC101 | ? | - |
? | |
additional information | large substrates have no ready access to the catalytic center | Phanerodontia chrysosporium OGC101 | ? | - |
? | |
additional information | enzyme oxidizes 2,6-dimethoxyphenol | Phanerodontia chrysosporium OGC101 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Phanerodontia chrysosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | one iron protoporphyrin IX prosthetic group per enzyme molecule | Phanerodontia chrysosporium | |
heme | enzyme has a single manganese binding site near the heme | Phanerodontia chrysosporium |