Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-hydroxy acid | activates by chelating and stabilizing Mn3+ rather than activating the enzyme | Phanerodontia chrysosporium | |
H2O2 | H2O2-dependent | Phanerodontia chrysosporium | |
Lactate | stimulates by complexing with and stabilizing Mn3+ | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
Mn2+ | - |
Phanerodontia chrysosporium | |
0.14 | - |
H2O2 | - |
Phanerodontia chrysosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Phanerodontia chrysosporium | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
- |
Phanerodontia chrysosporium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | involved in lignin-degradation, the mechanism enables the fungus to oxidize structures within woods which are inaccessible to enzymes | Mn3+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
white rot basidomycete | - |
Purification (Comment) | Organism |
---|---|
- |
Phanerodontia chrysosporium |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 Mn(II) + 2 H+ + H2O2 = 2 Mn(III) + 2 H2O | mechanism | Phanerodontia chrysosporium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
180 | - |
Mn3+-lactate complex formation | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Co2+ + H+ + H2O2 | reduction of enzyme compound II, oxidation at 2% the rate of Mn2+ oxidation | Phanerodontia chrysosporium | Co3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ complexed to lactate or other alpha-hydroxy acids acts as an obligatory oxidation intermediate in the oxidation of various dyes and lignin model compounds, Mn3+-lactate complex oxidizes all dyes oxidized by the enzyme in presence of Mn2+: NADH, pinacyanol, phenol red and poly B-411 | ? | |
Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | the diffusible product is Mn3+ | ? | |
Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | chelation of Mn3+ by organic acids stabilizes Mn3+ at a high redox potential | ? | |
Mn2+ + H+ + H2O2 | in presence of H2O2 enzyme oxidizes Mn2+ significantly faster than all other substrates, main function of enzyme is oxidation of Mn2+ to Mn3+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ complexed to lactate or other alpha-hydroxy acids acts as an obligatory oxidation intermediate in the oxidation of various dyes and lignin model compounds, Mn3+-lactate complex oxidizes all dyes oxidized by the enzyme in presence of Mn2+: NADH, pinacyanol, phenol red and poly B-411 | ? | |
Mn2+ + H+ + H2O2 | in presence of H2O2 enzyme oxidizes Mn2+ significantly faster than all other substrates, main function of enzyme is oxidation of Mn2+ to Mn3+ | Phanerodontia chrysosporium | Mn3+ + H2O | the diffusible product is Mn3+ | ? | |
Mn2+ + H+ + H2O2 | in presence of H2O2 enzyme oxidizes Mn2+ significantly faster than all other substrates, main function of enzyme is oxidation of Mn2+ to Mn3+ | Phanerodontia chrysosporium | Mn3+ + H2O | chelation of Mn3+ by organic acids stabilizes Mn3+ at a high redox potential | ? | |
Mn2+ + H+ + H2O2 | involved in lignin-degradation, the mechanism enables the fungus to oxidize structures within woods which are inaccessible to enzymes | Phanerodontia chrysosporium | Mn3+ + H2O | - |
? | |
additional information | enzyme oxidizes a variety of organic compounds in presence, but not in absence of Mn2+ | Phanerodontia chrysosporium | ? | - |
? | |
additional information | in absence of Mn2+ the enzyme oxidizes pinacyanol as most easily oxidized dye at 1.7% of the rate of the Mn2+ oxidation | Phanerodontia chrysosporium | ? | - |
? | |
additional information | enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Phanerodontia chrysosporium | ? | - |
? | |
additional information | in absence of H2O2 the enzyme oxidizes Mn-dependently NADH to NAD+, generating H2O2 for oxidizing other substrates | Phanerodontia chrysosporium | ? | - |
? | |
additional information | Mn2+-dependent oxidation of 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Phanerodontia chrysosporium | ? | - |
? | |
additional information | in absence of H2O2 the enzyme oxidizes Mn-dependently NADPH+ to NADP+ | Phanerodontia chrysosporium | ? | - |
? | |
additional information | enzyme oxidizes phenol red | Phanerodontia chrysosporium | ? | - |
? | |
additional information | enzyme oxidizes the polymeric dyes poly R-481 and poly B-411 | Phanerodontia chrysosporium | ? | - |
? | |
additional information | in presence of Mn2+ enzyme oxidizes various organic compounds | Phanerodontia chrysosporium | ? | - |
? | |
additional information | no oxidation of Ni2+ | Phanerodontia chrysosporium | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
oxidation of 2,2-azino-bis-3-ethyl-6-benzothiazolinesulfonate | Phanerodontia chrysosporium |
5 | - |
Mn3+-lactate complex formation | Phanerodontia chrysosporium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 5.5 | about half-maximal activity at pH 4.0 and 5.5, 2,2-azino-bis-3-ethyl-6-benzothiazolinesulfonate-oxidation | Phanerodontia chrysosporium |
4.5 | 5.7 | about 65% of maximal activity at pH 4.5 and about 50% at pH 5.7, Mn(III)-lactate formation | Phanerodontia chrysosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | heme protein | Phanerodontia chrysosporium |