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Literature summary for 1.11.1.11 extracted from

  • Kovacs, F.A.; Sarath, G.; Woodworth, K.; Twigg, P.; Tobias, C.M.
    Abolishing activity against ascorbate in a cytosolic ascorbate peroxidase from switchgrass (2013), Phytochemistry, 94, 45-52.
    View publication on PubMed
Search for more literature for 1.11.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. Expression of holo enzyme depends on the simultaneous production of protein and heme by the bacteria Panicum virgatum

Protein Variants

Protein Variants Comment Organism
R172S negligible ascorbate peroxidase activity, but shows near wild type activity toward other aromatic substrates Panicum virgatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000043
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 7.0, temperature not specified in the publication. Hill coefficient n is 1.0 Panicum virgatum
0.804
-
 L-ascorbate pH 7.0, temperature not specified in the publication. Non-Michaelis-Menten kinetics, Hill ccoefficient n is 1.6 Panicum virgatum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Panicum virgatum 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 1 * 26000, SDS-PAGE, His-tagged protein Panicum virgatum
29500
-
 gel filtration, His-tagged protein Panicum virgatum

Organism

Organism UniProt Comment Textmining
Panicum virgatum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-ascorbate + H2O2 + 2 H+
-
 Panicum virgatum 2 monodehydroascorbate + 2 H2O
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
 Panicum virgatum ? + H2O
-
 ?
additional information guaiacol and pyrogallol are substrates, but the enzyme is inactivated by the oxidized guaiacol and pyrogallol products Panicum virgatum ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 26000, SDS-PAGE, His-tagged protein Panicum virgatum

Synonyms

Synonyms Comment Organism
Pavirv00022559m
-
 Panicum virgatum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 7.0, temperature not specified in the publication. Hill coefficient n is 1.0 Panicum virgatum
161
-
 L-ascorbate pH 7.0, temperature not specified in the publication. Non-Michaelis-Menten kinetics, Hill ccoefficient n is 1.6 Panicum virgatum