Protein Variants | Comment | Organism |
---|---|---|
W208F | the optical spectrum of the W208F mutant closely resembles that of wild type LmAPX at pH 7.5 in the absence of ascorbate. W208F mutant causes a spectral red shift from high spin to low spin, indicating that the mutant can react with H2O2. Cytochrome c binding affinity to the enzyme does not alter after mutation. The mutant is 1000times less active than the wild type in cytochrome c oxidation | Leishmania major |
W208Y | mutant shows low spin hem. The mutant does not react with H2O2 | Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
guaiacol | 25°C, mutant enzyme W208F | Leishmania major | |
0.025 | - |
cytochrome c | 25°C, mutant enzyme W208F | Leishmania major | |
6 | - |
cytochrome c | 25°C, wild-type enzyme | Leishmania major | |
6.7 | - |
guaiacol | 25°C, wild-type enzyme | Leishmania major |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzyme W208F expressed in Escherichia coli | Leishmania major |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cytochrome c + H2O | - |
Leishmania major | ? | - |
? | |
guaiacol + H2O2 | - |
Leishmania major | ? | - |
? | |
L-ascorbate + H2O2 | - |
Leishmania major | dehydroascorbate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LmAPX | - |
Leishmania major |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
ascorbate | 25°C, wild-type enzyme | Leishmania major | |
1.83 | - |
cytochrome c | 25°C, wild-type enzyme | Leishmania major | |
2.5 | - |
guaiacol | 25°C, wild-type enzyme | Leishmania major |