Protein Variants | Comment | Organism |
---|---|---|
H42A | inactive, partial recovery of activity by addition of exogenous imidazoles | Pisum sativum |
H42E | decrease in kcat- and Km-value | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
L-ascorbate | pH 7.0, 25°C, mutant H42E | Pisum sativum | |
0.41 | - |
L-ascorbate | pH 7.0, 25°C, wild-type | Pisum sativum | |
2.9 | - |
guaiacol | pH 7.0, 25°C, mutant H42E | Pisum sativum | |
12.3 | - |
guaiacol | pH 7.0, 25°C, wild-type | Pisum sativum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Pisum sativum | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | predominantly five-coordinate high-spin iron | Pisum sativum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 L-ascorbate + H2O2 + 2 H+ = 2 monodehydroascorbate + 2 H2O | mechanism involves accumulation of a transient enzyme intermediate | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guaiacol + H2O2 | - |
Pisum sativum | ? + H2O | - |
? | |
L-ascorbate + H2O2 | - |
Pisum sativum | ? + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
guaiacol | pH 7.0, 25°C, mutant H42E | Pisum sativum | |
3.9 | - |
L-ascorbate | pH 7.0, 25°C, mutant H42E | Pisum sativum | |
66 | - |
guaiacol | pH 7.0, 25°C, wild-type | Pisum sativum | |
248 | - |
L-ascorbate | pH 7.0, 25°C, wild-type | Pisum sativum |