Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, most of the enzyme produced is present in the apo-form, without heme | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
H2O2 | recombinant enzyme | Glycine max | |
0.35 | - |
ascorbate | recombinant enzyme | Glycine max | |
1.8 | - |
guaiacol | recombinant enzyme | Glycine max | |
2.4 | - |
pyrogallol | recombinant enzyme | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Glycine max | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ascorbate + H2O2 | Glycine max | physiological role of the enzyme: removal of H2O2, prevention of H2O2 accumulation | dehydroascorbate + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
soybean | - |
Purification (Comment) | Organism |
---|---|
using Ni2+-agarose affinity chromatography and gel filtration after reconstitution with hemin | Glycine max |
Renatured (Comment) | Organism |
---|---|
the apo-recombinant protein is easily converted to the holo-recombinant form by in vitro reconstitution with hemin | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | nodules | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
effects of media amendments | Glycine max |
3 | 4 | affinity purified preparation | Glycine max |
31.7 | - |
purified recombinant enzyme | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-di-(3-ethyl-benzothiazoline-(6)-sulfonic acid) + H2O2 | 3% relative activity to L-ascorbate | Glycine max | ? + H2O | - |
? | |
guaiacol + H2O2 | 30.5% activity relative to L-ascorbate | Glycine max | ? | - |
? | |
L-ascorbate + H2O2 | - |
Glycine max | dehydroascorbate + H2O | - |
? | |
L-ascorbate + H2O2 | physiological role of the enzyme: removal of H2O2, prevention of H2O2 accumulation | Glycine max | dehydroascorbate + 2 H2O | - |
? | |
pyrogallol + H2O2 | 723% activity relative to L-ascorbate | Glycine max | 3-hydroxybenzo-1,2-quinone + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | 7 | - |
Glycine max |
7 | - |
assay at | Glycine max |