Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NAD+ | prevents formation of unstable intermediate of the enzyme and lowers Km for H2O2 | Enterococcus faecalis |
General Stability | Organism |
---|---|
25°C, without H2O2 in assay system 85% loss of activity in 30 min | Enterococcus faecalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | NADH converts the enzyme to an unstable intermediate that decays to inactive enzyme at suboptimal H2O2 concentrations | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0068 | - |
NADH | - |
Enterococcus faecalis | |
0.02 | - |
H2O2 | - |
Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
2 * 60000, calculation from FAD content | Enterococcus faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H2O2 | - |
Enterococcus faecalis | NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 60000, calculation from FAD content | Enterococcus faecalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
66.7 | - |
H2O2 | calculated per flavin | Enterococcus faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Enterococcus faecalis |