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Literature summary for 1.11.1.1 extracted from

  • Dolin, M.I.
    The Streptococcus faecalis oxidase for reduced diphosphopyridine nucleotide. III. Isolation and properties of a flavin peroxidase for reduced diphosphopyridine nucleotide (1957), J. Biol. Chem., 225, 557-573.
    View publication on PubMed
Search for more literature for 1.11.1.1

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 98% inhibition at 0.01 mM Enterococcus faecalis
Cl- strong inhibitor Enterococcus faecalis
Co2+ 25% inhibition at 1 mM Enterococcus faecalis
Cu2+ 85% inhibition at 1 mM Enterococcus faecalis
H2O2 43% inhibition at 16.5 mM Enterococcus faecalis
Hg2+ 21% inhibition at 0.01 mM Enterococcus faecalis
NaN3 15% inhibition at 50 mM Enterococcus faecalis
p-chloromercuribenzoate 20% inhibition at 0.33 mM Enterococcus faecalis
Pb2+ 49% inhibition at 0.33 mM Enterococcus faecalis
SO42- strong inhibitor Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 ferricyanide
-
 Enterococcus faecalis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe small amounts, significance unknown Enterococcus faecalis
additional information no Mo, Cu, Mn, Zn Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 calculation from FAD content Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H2O2 Enterococcus faecalis
-
 NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Enterococcus faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 8500-9000 U/ml, calculated as change of 0.01 of optical density Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ferricyanide + H2O2
-
 Enterococcus faecalis ferrocyanide + H2O
-
 ?
additional information NADH cannot be replaced by reduced cytochrome c, H2O2 cannot be replaced by Cu2+, Fe3+, SeO32+, NO3-, NO2-, oxidized glutathione, cystine, lipoic acid disulfide, dehydroascorbic acid, cytochrome c, FAD, flavin mononucleotide, riboflavin, 2,6-dichlorophenolindophenol, methylene blue, brilliant cresyl blue Enterococcus faecalis ?
-
 ?
NADH + H2O2
-
 Enterococcus faecalis NAD+ + H2O
-
 ?
NADH + H2O2 H2O2 can be replaced by 1,4-naphthoquinone which is one third as effective as H2O2 Enterococcus faecalis NAD+ + H2O
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
-
 Enterococcus faecalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
83.3
-
 NADH under standard assay conditions Enterococcus faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 ferricyanide Enterococcus faecalis
5.2 5.4 substrate H2O2 in acetate or phosphate buffer Enterococcus faecalis

Cofactor

Cofactor Comment Organism Structure
FAD FAD-flavoprotein, 1 mol FAD per 1 mol enzyme Enterococcus faecalis