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Literature summary for 1.10.5.1 extracted from

  • Wu, K.; Knox, R.; Sun, X.Z.; Joseph, P.; Jaiswal, A.K.; Zhang, D.; Deng, P.S.K.; Chen, S.
    Catalytic properties of NAD(P)H:quinone oxidoreductase-2 (NQO2), a dihydronicotinamide riboside dependent oxidoreductase (1997), Arch. Biochem. Biophys., 347, 221-228.
    View publication on PubMed
Search for more literature for 1.10.5.1

Protein Variants

Protein Variants Comment Organism
additional information construction of a chimeric enzyme of the human NQO2 with 43 amino acids from the carboxyl terminus of human DT-diaphorase. HNQO2-hDT43 still uses dihydronicotinamide riboside as an electron donor. The chimeric enzyme is inhibited by quercetin but not dicoumarol Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-hydroxyestradiol 0.01 mM, 17% inhibition Homo sapiens
4',5,7-trihydroxyflavone 0.01 mM, 61% inhibition Homo sapiens
4-hydroxyestradiol 0.01 mM, 18% inhibition Homo sapiens
4-hydroxyestrone 0.01 mM, 22% inhibition Homo sapiens
7,8-dihydroxyflavone 0.01 mM, 7% inhibition Homo sapiens
alpha-naphthoflavone 0.01 mM, 75% inhibition Homo sapiens
beta-naphthoflavone 0.01 mM, 76% inhibition Homo sapiens
chrysin 0.01 mM, complete inhibition Homo sapiens
dicoumarol 0.01 mM, 25% inhibition Homo sapiens
estradiol 0.01 mM, 13% inhibition Homo sapiens
flavone 0.01 mM, 14% inhibition Homo sapiens
galangin 0.01 mM, complete inhibition Homo sapiens
morin 0.01 mM, 95% inhibition Homo sapiens
quercetin 0.01 mM, complete inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 menadione/3-(4,5-dimethylthiazaol-2-yl)-2,5-diphenyltetrazolium
-
 Homo sapiens
0.0047
-
 Methyl red
-
 Homo sapiens
0.007
-
 2,6-dichlorophenolindophenol
-
 Homo sapiens
0.012
-
 3-(4,5-dimethylthiazaol-2-yl)-2,5-diphenyltetrazolium
-
 Homo sapiens
0.019
-
 2,6-dichlorophenolindophenol chimeric enzyme of the human NQO2 with 43 amino acids from the carboxyl terminus of human DT-diaphorase Homo sapiens
0.028
-
 dihydronicotinamide riboside
-
 Homo sapiens
0.252
-
 NADH
-
 Homo sapiens
0.26
-
 CB 1954
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 2 * 26000, SDS-PAGE Homo sapiens
50000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydronicotinamide riboside + 2,6-dichlorophenolindophenol
-
 Homo sapiens nicotinamide riboside + reduced 2,6-dichlorophenolindophenol
-
 ?
dihydronicotinamide riboside + 3-(4,5-dimethylthiazaol-2-yl)-2,5-diphenyltetrazolium
-
 Homo sapiens nicotinamide riboside + ?
-
 ?
dihydronicotinamide riboside + CB 1954
-
 Homo sapiens nicotinamide riboside + ?
-
 ?
dihydronicotinamide riboside + menadione/3-(4,5-dimethylthiazaol-2-yl)-2,5-diphenyltetrazolium
-
 Homo sapiens nicotinamide riboside + ?
-
 ?
dihydronicotinamide riboside + methyl red
-
 Homo sapiens nicotinamide riboside + ?
-
 ?
additional information no activity with one-electron acceptors such as potassium ferricyanide Homo sapiens ?
-
 ?
NADH + 2,6-dichlorophenolindophenol
-
 Homo sapiens ?
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 26000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
NQO2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6
-
 NADH
-
 Homo sapiens
3.83
-
 Methyl red
-
 Homo sapiens
6
-
 CB 1954
-
 Homo sapiens
11
-
 3-(4,5-dimethylthiazaol-2-yl)-2,5-diphenyltetrazolium
-
 Homo sapiens
38.33
-
 menadione/3-(4,5-dimethylthiazaol-2-yl)-2,5-diphenyltetrazolium
-
 Homo sapiens
43.33
-
 dihydronicotinamide riboside
-
 Homo sapiens
46.67
-
 2,6-dichlorophenolindophenol
-
 Homo sapiens
91.8
-
 2,6-dichlorophenolindophenol chimeric enzyme of the human NQO2 with 43 amino acids from the carboxyl terminus of human DT-diaphorase Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD one prosthetic group per subunit Homo sapiens
NADH very poor electron donor Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000021
-
 quercetin
-
 Homo sapiens