Application | Comment | Organism |
---|---|---|
environmental protection | laccase can be efficiently used to decolorize synthetic dye and is a suitable candidate for the treatment of wastewater from industrial effluents | Coriolopsis trogii |
Cloned (Comment) | Organism |
---|---|
expression of lcc3 gene in Komagataella (i.e. Pichia) pastoris | Coriolopsis trogii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4, 30°C | Coriolopsis trogii | |
2.095 | - |
2,6-dimethoxyphenol | pH 4, 30°C | Coriolopsis trogii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Coriolopsis trogii | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | multicopper enzyme | Coriolopsis trogii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coriolopsis trogii | A0A140CWW4 | - |
- |
Coriolopsis trogii BAFC 463 | A0A140CWW4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Coriolopsis trogii | ? | - |
? | |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Coriolopsis trogii BAFC 463 | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Coriolopsis trogii | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Coriolopsis trogii BAFC 463 | ? | - |
? | |
additional information | the enzyme is capable of decolorizing between 50% and 100% of indigoid, triarylmethane, azoic and anthraquinonic synthetic dyes in the presence of the natural redox mediator acetosyringone within 2 h of incubation at pH 6 and 70°C | Coriolopsis trogii | ? | - |
? | |
additional information | the enzyme is capable of decolorizing between 50% and 100% of indigoid, triarylmethane, azoic and anthraquinonic synthetic dyes in the presence of the natural redox mediator acetosyringone within 2 h of incubation at pH 6 and 70°C | Coriolopsis trogii BAFC 463 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Coriolopsis trogii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 75 | maximum activity at 50°C, retaining more than 80% of activity at temperatures between 60°C and 75°C, but less than 55% of activity at temperatures below 40°C | Coriolopsis trogii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the enzyme retains 100% of its initial activity after 3 h of incubation | Coriolopsis trogii |
70 | - |
half-life: 45 min | Coriolopsis trogii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
329 | - |
2,6-dimethoxyphenol | pH 4, 30°C | Coriolopsis trogii | |
399 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4, 30°C | Coriolopsis trogii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Coriolopsis trogii |
4 | - |
substrate: 2,6-dimethoxyphenol | Coriolopsis trogii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | 25°C, no decrease of activity for at least 5 days | Coriolopsis trogii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
157 | - |
2,6-dimethoxyphenol | pH 4, 30°C | Coriolopsis trogii | |
1596 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4, 30°C | Coriolopsis trogii |