KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0172 | - |
2-dianisidine | pH 6.0, 25°C | Pleurotus ostreatus | |
0.0552 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 6.0, 25°C | Pleurotus ostreatus | |
4 | - |
guaiacol | pH 6.0, 25°C | Pleurotus ostreatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | the enzyme is a multi-copper enzyme | Pleurotus ostreatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
native PAGE and gel filtration | Pleurotus ostreatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pleurotus ostreatus | the enzyme catalyzes the oxidation of a wide range of compounds, including poly-phenols and anilines. The one electron oxidation of the reducing substrate occurs concomitantly with the four-electron reduction of molecular oxygen to water | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus ostreatus | - |
grown on tomato pomace, several isoyzmes | - |
Purification (Comment) | Organism |
---|---|
method development for one-step affinity chromatographic purifcation, 46fold purification on 1,4-butanediol diglycidyl ether affinity resin, analysis of affinity chromatographic matrices containing either urea, acetamide, ethanolamine, or iminodiacetic acid, and best with 1,4-butanediol diglycidyl ether, as affinity ligands for enzyme purification from fermentation broth | Pleurotus ostreatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | cultivation of Pleurotus ostreatus on tomato pomace medium | Pleurotus ostreatus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.8 | - |
purified native enzyme, pH 6.0, 25°C | Pleurotus ostreatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Pleurotus ostreatus | ? | - |
? | |
2-dianisidine + O2 | - |
Pleurotus ostreatus | ? | - |
? | |
guaiacol + O2 | - |
Pleurotus ostreatus | ? | - |
? | |
additional information | the enzyme catalyzes the oxidation of a wide range of compounds, including poly-phenols and anilines. The one electron oxidation of the reducing substrate occurs concomitantly with the four-electron reduction of molecular oxygen to water | Pleurotus ostreatus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000-55000, SDS-PAGE | Pleurotus ostreatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pleurotus ostreatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
guaiacol | pH 6.0, 25°C | Pleurotus ostreatus | |
20 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 6.0, 25°C | Pleurotus ostreatus | |
20.7 | - |
2-dianisidine | pH 6.0, 25°C | Pleurotus ostreatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Pleurotus ostreatus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0172 | - |
2-dianisidine | pH 6.0, 25°C | Pleurotus ostreatus | |
0.0552 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 6.0, 25°C | Pleurotus ostreatus | |
4 | - |
guaiacol | pH 6.0, 25°C | Pleurotus ostreatus |