Cloned (Comment) | Organism |
---|---|
gene lac2, DNA and amino acid sequence determination and analysis | Coprinus comatus |
genes lac1 and lac2, DNA and amino acid sequence determination and analysis, expression of Lac1 in Pichia pastoris | Coprinus comatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTT | complete inhibition at 5 mM | Coprinus comatus | |
NaN3 | complete inhibition at 5 mM | Coprinus comatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.5, 40°C, recombinant Lac1 | Coprinus comatus | |
0.034 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.5, 40°C, recombinant Lac1 | Coprinus comatus | |
4.317 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.5, 40°C, recombinant Lac1 | Coprinus comatus | |
7.611 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.5, 40°C, recombinant Lac1 | Coprinus comatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | slightly activating | Coprinus comatus | |
K+ | slightly activating | Coprinus comatus | |
NH4+ | slightly activating | Coprinus comatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coprinus comatus | H9C4A2 | lac2; gene lac2 | - |
Coprinus comatus | H9C4A3 | lac1; genes lac1 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme Lac1 from Pichia pastoris by ultrafiltration, anion exchange chromatography, ultrafiltration, and gel filtration | Coprinus comatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Coprinus comatus | ? | - |
? | |
2,6-dimethylphenol + O2 | - |
Coprinus comatus | ? | - |
? | |
4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Coprinus comatus | ? | - |
? | |
4 2,6-dimethylphenol + O2 | - |
Coprinus comatus | ? | - |
? | |
4 guaiacol + O2 | - |
Coprinus comatus | ? | - |
? | |
4 syringaldazine + O2 | - |
Coprinus comatus | ? | - |
? | |
guaiacol + O2 | - |
Coprinus comatus | ? | - |
? | |
additional information | the enzyme shows dye decolorization potential, overview | Coprinus comatus | ? | - |
? | |
syringaldazine + O2 | - |
Coprinus comatus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LAC1 | - |
Coprinus comatus |
Lac2 | - |
Coprinus comatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
with substrates syringaldazine, recombinant Lac1 | Coprinus comatus |
55 | - |
with substrates guaiacol, recombinant Lac1 | Coprinus comatus |
65 | - |
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), recombinant Lac1 | Coprinus comatus |
70 | - |
with substrate 2,6-dimethylphenol, recombinant Lac1 | Coprinus comatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.5, 40°C, recombinant Lac1 | Coprinus comatus | |
1.15 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.5, 40°C, recombinant Lac1 | Coprinus comatus | |
7.67 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.5, 40°C, recombinant Lac1 | Coprinus comatus | |
15.93 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.5, 40°C, recombinant Lac1 | Coprinus comatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), recombinant Lac1 | Coprinus comatus |
5.5 | - |
with substrate 2,6-dimethylphenol, recombinant Lac1 | Coprinus comatus |
6 | - |
with substrates guaiacol and syringaldazine, recombinant Lac1 | Coprinus comatus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.5, 40°C, recombinant Lac1 | Coprinus comatus | |
1.78 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.5, 40°C, recombinant Lac1 | Coprinus comatus | |
42.55 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.5, 40°C, recombinant Lac1 | Coprinus comatus | |
465.8 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.5, 40°C, recombinant Lac1 | Coprinus comatus |