Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 14% inhibition at 5 mM | Trametes polyzona | |
Co2+ | 28% inhibition at 5 mM | Trametes polyzona | |
Cr3+ | 23% inhibition at 5 mM | Trametes polyzona | |
Cu2+ | 19% inhibition at 5 mM | Trametes polyzona | |
DTT | complete inhibition at 1 mM | Trametes polyzona | |
Fe2+ | 95% inhibition at 5 mM | Trametes polyzona | |
L-cysteine | complete inhibition at 10 mM | Trametes polyzona | |
Mg2+ | 6% inhibition at 5 mM | Trametes polyzona | |
NaCl | 50% inhibition at 20 mM, the inhibitory effect of chloride ions is reversible | Trametes polyzona | |
NaN3 | complete inhibition at 0.1 mM | Trametes polyzona | |
Zn2+ | 19% inhibition at 5 mM | Trametes polyzona |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, temperature not specified in the publication | Trametes polyzona | |
0.503 | - |
2,6-dimethoxyphenol | pH 5.0, temperature not specified in the publication | Trametes polyzona | |
1.89 | - |
guaiacol | pH 5.0, temperature not specified in the publication | Trametes polyzona | |
4.08 | - |
catechol | pH 5.0, temperature not specified in the publication | Trametes polyzona | |
6.28 | - |
L-Dopa | pH 5.0, temperature not specified in the publication | Trametes polyzona |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Trametes polyzona | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
native enzyme, gel filtration | Trametes polyzona |
71000 | - |
1 * 71000, SDS-PAGE | Trametes polyzona |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 catechol + O2 | Trametes polyzona | - |
4 1,2-benzoquinone + 2 H2O | - |
? | |
4 catechol + O2 | Trametes polyzona WR710-1 | - |
4 1,2-benzoquinone + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes polyzona | - |
- |
- |
Trametes polyzona WR710-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 45fold from solid-state fermentation on peel from Citrus reticulata to homogeneity by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography, ultrafiltration, and gel filtration | Trametes polyzona |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | laccase from Trametes polyzona WR710-1 is produced under solid-state fermentation using the peel from the Tangerine orange (Citrus reticulata Blanco) as substrate | Trametes polyzona | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
60.8 | - |
purified native extracellular enzyme, pH 5.0, temperature not specified in the publication, substrate 2,6-dimethoxyphenol | Trametes polyzona |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trametes polyzona | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trametes polyzona WR710-1 | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Trametes polyzona | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Trametes polyzona WR710-1 | ? | - |
? | |
4 catechol + O2 | - |
Trametes polyzona | 4 1,2-benzoquinone + 2 H2O | - |
? | |
4 catechol + O2 | - |
Trametes polyzona WR710-1 | 4 1,2-benzoquinone + 2 H2O | - |
? | |
guaiacol + O2 | - |
Trametes polyzona | ? | - |
? | |
guaiacol + O2 | - |
Trametes polyzona WR710-1 | ? | - |
? | |
L-DOPA + O2 | - |
Trametes polyzona | ? | - |
? | |
L-DOPA + O2 | - |
Trametes polyzona WR710-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 71000, SDS-PAGE | Trametes polyzona |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified native enzyme, 60 min, completely stable up to | Trametes polyzona |
50 | - |
purified native enzyme, 60 min, 10% loss of activity | Trametes polyzona |
70 | - |
purified native enzyme, 60 min, inactivation | Trametes polyzona |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
38.2 | - |
2,6-dimethoxyphenol | pH 5.0, temperature not specified in the publication | Trametes polyzona | |
83.2 | - |
L-Dopa | pH 5.0, temperature not specified in the publication | Trametes polyzona | |
92.2 | - |
guaiacol | pH 5.0, temperature not specified in the publication | Trametes polyzona | |
125 | - |
catechol | pH 5.0, temperature not specified in the publication | Trametes polyzona | |
594 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, temperature not specified in the publication | Trametes polyzona |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2 | - |
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | Trametes polyzona |
4 | - |
with substrates L-DOPA, guaiacol, and catechol | Trametes polyzona |
5 | - |
with substrate 2,6-dimethoxyphenol | Trametes polyzona |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | stable within this range | Trametes polyzona |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.3 | - |
L-Dopa | pH 5.0, temperature not specified in the publication | Trametes polyzona | |
30.6 | - |
catechol | pH 5.0, temperature not specified in the publication | Trametes polyzona | |
48.7 | - |
guaiacol | pH 5.0, temperature not specified in the publication | Trametes polyzona | |
75.9 | - |
2,6-dimethoxyphenol | pH 5.0, temperature not specified in the publication | Trametes polyzona | |
3960 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, temperature not specified in the publication | Trametes polyzona |