Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme adsorption to cellulose fibers from cotton and denim, attachment of PEG of 1.1-5.0 kDa to the purified enzyme, overview | Trametes hirsuta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00134 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 4.5, 50°C, free enzyme | Trametes hirsuta | |
0.005 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 4.5, 50°C, enzyme conjugated with 1.1 kDa PEG | Trametes hirsuta | |
0.00632 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 4.5, 50°C, enzyme conjugated with 2 kDa PEG | Trametes hirsuta | |
0.00704 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 4.5, 50°C, enzyme conjugated with 5 kDa PEG | Trametes hirsuta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
free enzyme, gel filtration | Trametes hirsuta |
85000 | - |
enzyme conjugated with 1.1 kDa PEG, gel filtration | Trametes hirsuta |
177000 | - |
enzyme conjugated with 2 kDa PEG, gel filtration | Trametes hirsuta |
269000 | - |
enzyme conjugated with 5 kDa PEG, gel filtration | Trametes hirsuta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes hirsuta | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Trametes hirsuta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Trametes hirsuta | ? | - |
? | |
additional information | substrate specificities of a laccase chemically modified by attachment of PEG on soluble and cellulose-bound dye substrates, overview | Trametes hirsuta | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52000, SDS-PAGE | Trametes hirsuta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Trametes hirsuta |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified free enzyme half-life: 140 min, purified enzyme conjugated with 1.1 kDa PEG half-life: 70 min, purified enzyme conjugated with 2 kDa PEG half-life: 150 min, purified enzyme conjugated with 5.0 kDa PEG half-life: 210 min | Trametes hirsuta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Trametes hirsuta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6.5 | - |
Trametes hirsuta |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 6.5 | stable, purified enzyme | Trametes hirsuta |