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Literature summary for 1.10.3.11 extracted from

  • Berthold, D.A.
    Isolation of mutants of the Arabidopsis thaliana alternative oxidase (ubiquinol:oxygen oxidoreductase) resistant to salicylhydroxamic acid (1998), Biochim. Biophys. Acta, 1364, 73-83.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
pyruvate the alternative oxidase is 50% activated at a concentration of 0.1 mM pyruvate Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes are expressed in heme-deficient Escherichia coli SASX41B cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
F215L the mutant exhibits 1.6fold resistance to salicylhydroxamic acid compared to the wild type enzyme Arabidopsis thaliana
G303E the mutant exhibits 4.6fold resistance to salicylhydroxamic acid compared to the wild type enzyme Arabidopsis thaliana
M219I the mutant exhibits 1.4fold resistance to salicylhydroxamic acid compared to the wild type enzyme Arabidopsis thaliana
M219V the mutant exhibits 1.7fold resistance to salicylhydroxamic acid compared to the wild type enzyme Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
propyl gallate
-
Arabidopsis thaliana
Salicylhydroxamic acid
-
Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial membrane
-
Arabidopsis thaliana 31966
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme contains non-heme iron at its active site Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
duroquinol + O2
-
Arabidopsis thaliana duroquinone + H2O
-
?

Synonyms

Synonyms Comment Organism
alternative oxidase
-
Arabidopsis thaliana
ubiquinol:oxygen oxidoreductase
-
Arabidopsis thaliana

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00036
-
mutant enzyme F215L, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana propyl gallate
0.00039
-
mutant enzyme M219V, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana propyl gallate
0.00039
-
wild type enzyme, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana propyl gallate
0.00042
-
mutant enzyme M219I, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana propyl gallate
0.00044
-
mutant enzyme G303E, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana propyl gallate
0.042
-
wild type enzyme, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana Salicylhydroxamic acid
0.057
-
mutant enzyme M219I, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana Salicylhydroxamic acid
0.069
-
mutant enzyme F215L, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana Salicylhydroxamic acid
0.073
-
mutant enzyme M219V, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana Salicylhydroxamic acid
0.194
-
mutant enzyme G303E, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana Salicylhydroxamic acid