Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyruvate | the alternative oxidase is 50% activated at a concentration of 0.1 mM pyruvate | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
mutant enzymes are expressed in heme-deficient Escherichia coli SASX41B cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
F215L | the mutant exhibits 1.6fold resistance to salicylhydroxamic acid compared to the wild type enzyme | Arabidopsis thaliana |
G303E | the mutant exhibits 4.6fold resistance to salicylhydroxamic acid compared to the wild type enzyme | Arabidopsis thaliana |
M219I | the mutant exhibits 1.4fold resistance to salicylhydroxamic acid compared to the wild type enzyme | Arabidopsis thaliana |
M219V | the mutant exhibits 1.7fold resistance to salicylhydroxamic acid compared to the wild type enzyme | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
propyl gallate | - |
Arabidopsis thaliana | |
Salicylhydroxamic acid | - |
Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial membrane | - |
Arabidopsis thaliana | 31966 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains non-heme iron at its active site | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
duroquinol + O2 | - |
Arabidopsis thaliana | duroquinone + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alternative oxidase | - |
Arabidopsis thaliana |
ubiquinol:oxygen oxidoreductase | - |
Arabidopsis thaliana |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00036 | - |
mutant enzyme F215L, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C | Arabidopsis thaliana | propyl gallate | |
0.00039 | - |
mutant enzyme M219V, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C | Arabidopsis thaliana | propyl gallate | |
0.00039 | - |
wild type enzyme, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C | Arabidopsis thaliana | propyl gallate | |
0.00042 | - |
mutant enzyme M219I, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C | Arabidopsis thaliana | propyl gallate | |
0.00044 | - |
mutant enzyme G303E, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C | Arabidopsis thaliana | propyl gallate | |
0.042 | - |
wild type enzyme, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C | Arabidopsis thaliana | Salicylhydroxamic acid | |
0.057 | - |
mutant enzyme M219I, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C | Arabidopsis thaliana | Salicylhydroxamic acid | |
0.069 | - |
mutant enzyme F215L, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C | Arabidopsis thaliana | Salicylhydroxamic acid | |
0.073 | - |
mutant enzyme M219V, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C | Arabidopsis thaliana | Salicylhydroxamic acid | |
0.194 | - |
mutant enzyme G303E, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C | Arabidopsis thaliana | Salicylhydroxamic acid |