Cloned (Comment) | Organism |
---|---|
expressed in heme-deficient Escherichia coli BL21(DE3) cells | Trypanosoma brucei brucei |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 28-34% (w/v) PEG 400, 100 mM imidazole buffer pH 7.4, 500 mM potassium formate and 0.4% (w/v) C8E4l | Trypanosoma brucei brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascofuranone | specifically inhibits the quinol oxidase activity of TAO | Trypanosoma brucei brucei | |
additional information | insensitive to cyanide | Trypanosoma brucei brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial membrane | - |
Trypanosoma brucei brucei | 31966 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Trypanosoma brucei brucei |
110000 | - |
gel filtration | Trypanosoma brucei brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei brucei | - |
- |
- |
Trypanosoma brucei brucei FN102 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ultracentrifugation and Talon Co2+-affinity column chromatography | Trypanosoma brucei brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ubiquinol + O2 | a non-proton-pumping ubiquinol oxidase that catalyzes the fourelectron reduction of dioxygen to water | Trypanosoma brucei brucei | ubiquinone + H2O | - |
? | |
ubiquinol + O2 | a non-proton-pumping ubiquinol oxidase that catalyzes the fourelectron reduction of dioxygen to water | Trypanosoma brucei brucei FN102 | ubiquinone + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 38000, SDS-PAGE | Trypanosoma brucei brucei |
Synonyms | Comment | Organism |
---|---|---|
AOX | - |
Trypanosoma brucei brucei |
cyanide-insensitive alternative oxidase | - |
Trypanosoma brucei brucei |
TAO | - |
Trypanosoma brucei brucei |