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Literature summary for 1.10.3.1 extracted from

  • Liu, N.N.; Liu, W.; Wang, D.J.; Zhou, Y.B.; Lin, X.J.; Wang, X.; Li, S.B.
    Purification and partial characterization of polyphenol oxidase from the flower buds of Lonicera japonica Thunb (2013), Food Chem., 138, 478-483.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
SDS 170% activity at 10 mM Lonicera japonica

Inhibitors

Inhibitors Comment Organism Structure
ascorbic acid nearly complete inhibition at 10 mM Lonicera japonica
Citric acid 32% residual activity at 10 mM Lonicera japonica
Co2+ 35% residual activity at 10 mM Lonicera japonica
Cu2+ 33% residual activity at 10 mM Lonicera japonica
kojic acid 11% residual activity at 10 mM Lonicera japonica
Mg2+ inhibits activity at 0.01 mM Lonicera japonica
Mn2+ inhibits activity at 0.01 mM Lonicera japonica
additional information not inhibited by SDS Lonicera japonica
Sodium metabisulfite 27% residual activity at 10 mM Lonicera japonica
tropolone 34% residual activity at 10 mM Lonicera japonica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.213
-
catechol at pH 7.5 and 25°C Lonicera japonica
1
-
chlorogenic acid at pH 7.5 and 25°C Lonicera japonica
2.69
-
L-Dopa at pH 7.5 and 25°C Lonicera japonica

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 124% activity at 10 mM Lonicera japonica
copper a copper-containing enzyme with a dinuclear copper centre Lonicera japonica
Mg2+ stimulates activity to 112% at 10 mM Lonicera japonica
Mn2+ stimulates activity to 120% at 10 mM Lonicera japonica
additional information Na+, K+, Cl- and SO42- have no effect on enzyme activity Lonicera japonica
Zn2+ 122% activity at 0.1 mM Lonicera japonica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
1 * 49000, SDS-PAGE Lonicera japonica
49277
-
1 * 49277, LC-ESI mass spectrometry Lonicera japonica

Organism

Organism UniProt Comment Textmining
Lonicera japonica
-
variety Yate
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and DEAE-cellulose column chromatography Lonicera japonica

Source Tissue

Source Tissue Comment Organism Textmining
flower bud
-
Lonicera japonica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.145
-
using L-DOPA as substrate, crude enzyme, at pH 7.5 and 25°C Lonicera japonica
4.458
-
using L-DOPA as substrate, after 30.76fold purification, at pH 7.5 and 25°C Lonicera japonica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
catechol + O2 best substrate Lonicera japonica ?
-
?
chlorogenic acid + O2
-
Lonicera japonica ?
-
?
L-DOPA + O2
-
Lonicera japonica ?
-
?
additional information polyphenol oxidase shows no activity with L-tyrosine, digallol, gallic acid, and methyl gallate Lonicera japonica ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 49000, SDS-PAGE Lonicera japonica
monomer 1 * 49277, LC-ESI mass spectrometry Lonicera japonica

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
Lonicera japonica
PPO
-
Lonicera japonica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Lonicera japonica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
the residual soluble enzyme activity decreases significantly when temperature reaches 40°C Lonicera japonica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Lonicera japonica

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 the enzyme is stable within pH 8.0-10.0, retaining more than 80% of its activity. The enzyme is almost unstable and inactive when pH is below 3.0, only remaining less than 20% of its activity Lonicera japonica