Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-hydroxy-2,4,6-cycloheptatrien-1-one | 1 mM, complete inhibition of isoenzymes Ia, Ib and II | Portulaca grandiflora | |
4-chloromercuribenzoate | 1 mM, 96, 94 and 95% inhibition of isoenzymes Ia, Ib and II respectively | Portulaca grandiflora | |
ascorbic acid | - |
Portulaca grandiflora | |
Fe2+ | 88%, 68% and 80% inhibition of isoenzymes Ia, Ib, and II, respectively | Portulaca grandiflora | |
glutathione | 1 mM, 98%, 95% and 96% inhibition of isoenzymes Ia, Ib, and II, respectively | Portulaca grandiflora | |
iodoacetate | 1 mM; 1 mM: 46%, 39% and 31% inhibition of isoenzymes Ia, Ib, and II, respectively | Portulaca grandiflora | |
isoascorbic acid | 1 mM, complete inhibition of isoenzymes Ia, Ib and II | Portulaca grandiflora | |
kojic acid | 1 mM complete inhibition of isoenzymes Ia, Ib and II | Portulaca grandiflora | |
mimosine | 1 mM, 88%, 79% and 82% inhibition of isoenzymes Ia, Ib, and II, respectively | Portulaca grandiflora | |
additional information | not inhibited by 2,2'-dipyridyl, 1,10-phenanthroline and EDTA | Portulaca grandiflora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
L-Dopa | isoenzyme Ia | Portulaca grandiflora | |
2.2 | - |
L-Dopa | isoenzyme Ib | Portulaca grandiflora | |
3.5 | - |
L-Dopa | isoenzyme II | Portulaca grandiflora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
1 * 27000 + 1 * 25000, isoenzyme II, SDS-PAGE | Portulaca grandiflora |
27000 | - |
1 * 27000 + 1 * 25000, isoenzyme II, SDS-PAGE | Portulaca grandiflora |
53000 | - |
isoenzyme II, gel filtration | Portulaca grandiflora |
65000 | - |
isoenzymes Ia and Ib, gel filtration | Portulaca grandiflora |
66000 | - |
1 * 66000, isoenzyme Ia, SDS-PAGE | Portulaca grandiflora |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Portulaca grandiflora | - |
isoenzymes Ia, Ib and II | - |
Purification (Comment) | Organism |
---|---|
isoenzymes Ia, Ib and II ammonium sulfate, DEAE-cellulose, Sephacryl S-200, hydroxyapatite, Mono Q | Portulaca grandiflora |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
callus | cell culture | Portulaca grandiflora | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
isoenzyme Ia, 123 units/mg, isoenzyme Ib, 113 units/mg, isoenzyme II, 242 units/mg, 1 unit is defined as absorbance change at 420 nm of 0.1 per min | Portulaca grandiflora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + 1/2 O2 | isoenzymes Ia, Ib and II, 90, 43 and 42% of activity with L-dopa respectively, no activity with L-tyrosine, D-tyrosine, hydroquinone and methylhydroquinone | Portulaca grandiflora | 1,2-benzoquinone + H2O | - |
? | |
D-dopa + 1/2 O2 | isoenzymes Ia, Ib and II, 54, 60 and 48% of activity with L-dopa respectively | Portulaca grandiflora | D-dopaquinone + H2O | - |
? | |
L-dopa + 1/2 O2 | - |
Portulaca grandiflora | L-dopaquinone + H2O | - |
? | |
pyrogallol + O2 | isoenzymes Ia, Ib and II, 5, 8 and 15% of activity with L-dopa respectively | Portulaca grandiflora | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 27000 + 1 * 25000, isoenzyme II, SDS-PAGE | Portulaca grandiflora |
monomer | 1 * 65000, isoenzyme Ib, SDS-PAGE | Portulaca grandiflora |
monomer | 1 * 66000, isoenzyme Ia, SDS-PAGE | Portulaca grandiflora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
isoenzymes Ia and Ib | Portulaca grandiflora |
6 | - |
isoenzyme II | Portulaca grandiflora |