Any feedback?
Literature summary for 1.1.99.B3 extracted from
- Fusion of a xylan-binding module to gluco-oligosaccharide oxidase increases activity and promotes stable immobilization (2014), PLoS ONE, 9, e95170.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris strain KM71H | Sarocladium strictum |
General Stability
| General Stability | Organism |
|---|---|
| the presence of the xylan-binding module CBM22A of Clostridium thermocellum fused to the N-terminal of the enzyme increases its catalytic activity on mono- and oligo-saccharides by 2-3fold while not affecting binding affinity to these substrates, does not alter the thermostability of the enzyme or reduces substrate inhibition | Sarocladium strictum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
- |
Sarocladium strictum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sarocladium strictum | D7PF15 | - |
- |
| Sarocladium strictum CBS 346.70 | D7PF15 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Sarocladium strictum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography | Sarocladium strictum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + O2 | - |
Sarocladium strictum | cellobiono-1,5-lactone + H2O2 | - |
? |  |
| cellobiose + O2 | - |
Sarocladium strictum CBS 346.70 | cellobiono-1,5-lactone + H2O2 | - |
? | |
| cellohexaose + O2 | - |
Sarocladium strictum | cellohexaono-1,5-lactone + H2O2 | - |
? | |
| cellohexaose + O2 | - |
Sarocladium strictum CBS 346.70 | cellohexaono-1,5-lactone + H2O2 | - |
? | |
| cellopentaose + O2 | - |
Sarocladium strictum | cellopentaono-1,5-lactone + H2O2 | - |
? | |
| cellopentaose + O2 | - |
Sarocladium strictum CBS 346.70 | cellopentaono-1,5-lactone + H2O2 | - |
? | |
| cellotriose + O2 | - |
Sarocladium strictum | cellotriono-1,5-lactone + H2O2 | - |
? | |
| cellotriose + O2 | - |
Sarocladium strictum CBS 346.70 | cellotriono-1,5-lactone + H2O2 | - |
? | |
| D-glucose + O2 | - |
Sarocladium strictum | D-glucono-1,5-lactone + H2O2 | - |
? | |
| D-glucose + O2 | - |
Sarocladium strictum CBS 346.70 | D-glucono-1,5-lactone + H2O2 | - |
? | |
| D-xylose + O2 | - |
Sarocladium strictum | D-xylono-1,5-lactone + H2O2 | - |
? | |
| debranched soluble oat spelt xylan + acceptor | - |
Sarocladium strictum | ? | - |
? | |
| soluble oat spelt xylan + acceptor | - |
Sarocladium strictum | ? | - |
? | |
| xylobiose + O2 | highest activity | Sarocladium strictum | xylobiono-1,5-lactone + H2O2 | - |
? | |
| xylohexaose + O2 | - |
Sarocladium strictum | xylohexaono-1,5-lactone + H2O2 | - |
? | |
| xylopentaose + O2 | - |
Sarocladium strictum | xylopentaono-1,5-lactone + H2O2 | - |
? | |
| xylotriose + O2 | - |
Sarocladium strictum | xylotriono-1,5-lactone + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 76000, calculated from amino acid sequence | Sarocladium strictum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gluco-oligosaccharide oxidase | - |
Sarocladium strictum |
| GOOX-VN | - |
Sarocladium strictum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
