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Literature summary for 1.1.99.B1 extracted from

  • Tazoe, M.; Ichikawa, K.; Hoshino, T.
    Flavin adenine dinucleotide-dependent 4-phospho-D-erythronate dehydrogenase is responsible for the 4-phosphohydroxy-L-threonine pathway in vitamin B6 biosynthesis in Sinorhizobium meliloti (2006), J. Bacteriol., 188, 4635-4645.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
SDS-PAGE Sinorhizobium meliloti
53190
-
predicted from the pdxR DNA sequence Sinorhizobium meliloti
106000
-
gel filtration chromatography Sinorhizobium meliloti

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti
-
formerly Rhizobium meliloti, strain IFO 14782
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose FF column chromatography and Sephadex LH 20 column chromatography Sinorhizobium meliloti

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.946
-
0.091 mM DCIP and 0.00024 mg purified enzyme in 33 mM potassium phosphate buffer at pH 7.0 Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-phospho-D-erythronate + 2,6-dichloroindophenol
-
Sinorhizobium meliloti 3-hydroxy-4-phosphohydroxy-alpha-ketobutyrate + reduced 2,6-dichloroindophenol
-
?
4-phospho-D-erythronate + FAD
-
Sinorhizobium meliloti 3-hydroxy-4-phosphohydroxy-alpha-ketobutyrate + FADH2
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 53000, SDS-PAGE Sinorhizobium meliloti

Synonyms

Synonyms Comment Organism
4PE dehydrogenase
-
Sinorhizobium meliloti
4PE oxidoreductase
-
Sinorhizobium meliloti
flavin adenine dinucleotide-dependent 4-phospho-D-erythronate dehydrogenase
-
Sinorhizobium meliloti
pdxR-encoded 4PE dehydrogenase
-
Sinorhizobium meliloti

Cofactor

Cofactor Comment Organism Structure
FAD
-
Sinorhizobium meliloti