Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-pyridoxic acid | - |
Arthrobacter sp. | |
Cu2+ | - |
Arthrobacter sp. | |
Dithionitrobenzoic acid | - |
Arthrobacter sp. | |
Hg2+ | - |
Arthrobacter sp. | |
Isopyridoxal | - |
Arthrobacter sp. | |
N-ethylmaleimide | - |
Arthrobacter sp. | |
Ni2+ | - |
Arthrobacter sp. | |
p-chloromercuribenzoate | - |
Arthrobacter sp. | |
pyridoxal | - |
Arthrobacter sp. | |
pyridoxamine | - |
Arthrobacter sp. | |
sulfhydryl reagents | - |
Arthrobacter sp. | |
Zn2+ | - |
Arthrobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
pyridoxine | - |
Arthrobacter sp. | |
0.28 | - |
O2 | - |
Arthrobacter sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Arthrobacter sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58600 | - |
2 * 58600, SDS-PAGE | Arthrobacter sp. |
112000 | - |
gel filtration | Arthrobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxine + acceptor | Arthrobacter sp. | utilization of pyridoxine as sole source of carbon and nitrogen | isopyridoxal + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
Cr-7, inducible enzyme | - |
Purification (Comment) | Organism |
---|---|
- |
Arthrobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.9 | - |
- |
Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-deoxypyridoxine + acceptor | 18% of activity with pyridoxine | Arthrobacter sp. | 3-hydroxy-2-methylpyridine-5-carboxaldehyde + reduced acceptor | - |
? | |
pyridoxal + acceptor | 29% of activity with pyridoxine | Arthrobacter sp. | 3-hydroxy-2-methylpyridine-4,5-dicarbaldehyde + reduced acceptor | - |
? | |
pyridoxamine + acceptor | 27% of activity with pyridoxine | Arthrobacter sp. | 4-aminomethyl-5-formyl-2-methylpyridin-3-ol + reduced acceptor | - |
? | |
pyridoxine + acceptor | NAD+, NADP+ | Arthrobacter sp. | isopyridoxal + reduced acceptor | - |
? | |
pyridoxine + acceptor | O2 or quinones | Arthrobacter sp. | isopyridoxal + reduced acceptor | - |
? | |
pyridoxine + acceptor | acceptor: 2,6-dichlorophenolindophenol | Arthrobacter sp. | isopyridoxal + reduced acceptor | - |
? | |
pyridoxine + acceptor | utilization of pyridoxine as sole source of carbon and nitrogen | Arthrobacter sp. | isopyridoxal + reduced acceptor | - |
? | |
pyridoxine + O2 | - |
Arthrobacter sp. | isopyridoxal + H2O2 | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 58600, SDS-PAGE | Arthrobacter sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
pyridoxine | acceptor O2 | Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.3 | pyridoxine + O2, Tris buffer, phosphate buffer | Arthrobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.4 | 9.5 | activity at pH 9.5 in Tris is about 70% of that at pH 7.3 while activities at pH 6.4 and pH 6.0 in phosphate buffer are about 75% and 0% respectively of that at pH 7.3 | Arthrobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein, FAD-dependent | Arthrobacter sp. |