Protein Variants | Comment | Organism |
---|---|---|
D448N | substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 72%, D-allose 39%, D-galactose 14%, D-maltose 36%, D-lactose 48%, respectively | Acinetobacter calcoaceticus |
D456N | substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 78%, D-allose 43%, D-galactose 16%, D-maltose 41%, D-lactose 59%, respectively | Acinetobacter calcoaceticus |
DD457N | substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 80%, D-allose 46%, D-galactose 11%, D-maltose 32%, D-lactose 43%, respectively | Acinetobacter calcoaceticus |
N452T | substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 59%, D-allose 33%, D-galactose 5%, D-maltose 30%, D-lactose 31%, respectively | Acinetobacter calcoaceticus |
N462H | substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 53%, D-allose 32%, D-galactose 2%, D-maltose 25%, D-lactose 31%, respectively | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
D-galactose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
3.7 | - |
D-galactose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
5.3 | - |
D-galactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
12.3 | - |
D-glucose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
12.5 | - |
D-glucose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
16 | - |
D-maltose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
18 | - |
D-lactose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
18.9 | - |
D-lactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
25 | - |
D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
26 | - |
D-maltose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
27.6 | - |
3-O-methyl-D-glucose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
28.7 | - |
3-O-methyl-D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
28.8 | - |
3-O-methyl-D-glucose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
32.5 | - |
D-allose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
33.6 | - |
D-lactose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
35.5 | - |
D-allose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
38.7 | - |
D-allose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
46.5 | - |
D-maltose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol | 81% of the activity with D-glucose | Acinetobacter calcoaceticus | 3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-allose + oxidized 2,6-dichlorophenolindophenol | 47% of the activity with D-glucose | Acinetobacter calcoaceticus | D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-galactose + oxidized 2,6-dichlorophenolindophenol | 11% of the activity with D-glucose | Acinetobacter calcoaceticus | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
lactose + oxidized 2,6-dichlorophenolindophenol | 61% of the activity with D-glucose | Acinetobacter calcoaceticus | lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltose + oxidized 2,6-dichlorophenolindophenol | 61% of the activity with D-glucose | Acinetobacter calcoaceticus | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PQQ glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
PQQGDH-B | - |
Acinetobacter calcoaceticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
69 | - |
D-galactose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
72 | - |
D-galactose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
232 | - |
D-galactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
574 | - |
D-lactose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
588 | - |
D-maltose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
949 | - |
D-allose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1002 | - |
D-maltose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1035 | - |
D-allose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1038 | - |
D-lactose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1064 | - |
3-O-methyl-D-glucose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1253 | - |
3-O-methyl-D-glucose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1399 | - |
D-glucose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1659 | - |
D-lactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1791 | - |
D-glucose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1930 | - |
D-maltose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
2509 | - |
D-allose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
3011 | - |
3-O-methyl-D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
3860 | - |
D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
D-maltose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
23 | - |
D-galactose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
25 | - |
D-allose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
26 | - |
D-galactose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
31 | - |
D-lactose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
32 | - |
D-allose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
32 | - |
D-lactose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
37 | - |
D-maltose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
37 | - |
3-O-methyl-D-glucose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
44 | - |
D-galactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
45 | - |
3-O-methyl-D-glucose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
71 | - |
D-allose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
74 | - |
D-maltose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
88 | - |
D-lactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
105 | - |
3-O-methyl-D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
114 | - |
D-glucose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
143 | - |
D-glucose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
154 | - |
D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus |