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Literature summary for 1.1.99.31 extracted from

  • Lehoux, I.E.; Mitra, B.
    (S)-Mandelate dehydrogenase from Pseudomonas putida: mechanistic studies with alternate substrates and pH and kinetic isotope effects (1999), Biochemistry, 38, 5836-5848.
    View publication on PubMed
Search for more literature for 1.1.99.31

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
(R,S)-2-hydroxy-3-butynoic acid irreversible inactivation Pseudomonas putida
(S)-1-phenyl-1,2-ethanediol competitive inhibitor Pseudomonas putida
(S)-2-methoxy-2-phenylacetate competitive inhibitor Pseudomonas putida
(S)-3-phenyllactate competitive inhibitor Pseudomonas putida
2-phenylacetate competitive inhibitor Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 (R,S)-mandelate in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C Pseudomonas putida
0.4
-
 (R,S)-indoleglycolate in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C Pseudomonas putida
0.75
-
 (R,S)-2-hydroxyoctanoate in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C Pseudomonas putida
0.9
-
 (R,S)-3-indolelactate in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C Pseudomonas putida
2
-
 DL-3-phenyllactate in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C Pseudomonas putida
4.3
-
 (R,S)-2-hydroxyisocaproate in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C Pseudomonas putida
4.9
-
 (R,S)-2-hydroxyhexanoate in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C Pseudomonas putida
9.5
-
 (R,S)-2-hydroxyisovalerate in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C Pseudomonas putida
15.3
-
 (R,S)-2-hydroxyvalerate in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C Pseudomonas putida
22
-
 (R,S)-2-hydroxy-3-butynoic acid in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C Pseudomonas putida
32
-
 (R,S)-2-hydroxybutyrate in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida P20932 strain ATCC 12633
-

Purification (Commentary)

Purification (Comment) Organism
His6-tagged enzyme, Ni-resin column chromatography and Sephadex G-25 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-2-hydroxy-3-butenoic acid + acceptor very low binding affinity for MDH Pseudomonas putida 2-oxo-3-butenoic acid + reduced acceptor
-
 ?
(R,S)-2-hydroxybutyrate + acceptor slow substrate Pseudomonas putida 2-oxobutyrate + reduced acceptor
-
 ?
(R,S)-2-hydroxybutyric acid + acceptor very low binding affinity for MDH Pseudomonas putida 2-oxo-3-butynoic acid + reduced acceptor
-
 ?
(R,S)-2-hydroxyhexanoate + acceptor
-
 Pseudomonas putida 2-oxohexanoate + reduced acceptor
-
 ?
(R,S)-2-hydroxyisocaproate + acceptor
-
 Pseudomonas putida 2-oxoisocaproate + reduced acceptor
-
 ?
(R,S)-2-hydroxyisovalerate + acceptor
-
 Pseudomonas putida 2-oxo-isovalerate + reduced acceptor
-
 ?
(R,S)-2-hydroxyoctanoate + acceptor slow substrate Pseudomonas putida 2-oxooctanoate + reduced acceptor
-
 ?
(R,S)-2-hydroxyvalerate + acceptor
-
 Pseudomonas putida 2-oxovalerate + reduced acceptor
-
 ?
(R,S)-3-indolelactate + acceptor
-
 Pseudomonas putida 3-(1H-indol-2-yl)-2-oxopropanoate + reduced acceptor
-
 ?
(R,S)-3-phenyllactate + 2,6-dichlorophenolindophenol slow substrate Pseudomonas putida phenylpyruvate + reduced 2,6-dichlorophenolindophenol
-
 ?
(R,S)-indoleglycolate + acceptor
-
 Pseudomonas putida indol-2-yl(oxo)acetate + reduced acceptor
-
 ?
(R,S)-mandelate + 2,6-dichlorophenolindophenol
-
 Pseudomonas putida 2-oxo-2-phenylacetate + reduced 2,6-dichlorophenolindophenol
-
 ?
(R,S)-mandelate + ferricyanide
-
 Pseudomonas putida 2-oxo-2-phenylacetate + ferrocyanide
-
 ?
2-hydroxy-3-butynoate + acceptor
-
 Pseudomonas putida 2-oxo-3-butynoate + reduced acceptor
-
 ?

Synonyms

Synonyms Comment Organism
MDH
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
36
-
 (R,S)-2-hydroxy-3-butynoic acid in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C Pseudomonas putida