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Literature summary for 1.1.99.31 extracted from

  • Smekal, O.; Yasin, M.; Fewson, C.A.; Reid, G.A.; Chapman, S.K.
    L-mandelate dehydrogenase from Rhodotorula graminis: comparisons with the L-lactate dehydrogenase (flavocytochrome b2) from Saccharomyces cerevisiae (1993), Biochem. J., 290, 103-107.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.99.31

Inhibitors

Inhibitors Comment Organism Structure
hexanoate
-
 Rhodotorula graminis
L-lactate
-
 Rhodotorula graminis
L-Phenyllactate
-
 Rhodotorula graminis
oxalate
-
 Rhodotorula graminis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 (S)-Mandelate pH 7.5, 25°C, electron acceptor: 2,6-dichloroindophenol Rhodotorula graminis
0.27
-
 (S)-Mandelate pH 7.5, 25°C, electron acceptor: ferricyanide Rhodotorula graminis
0.49
-
 (S)-Mandelate pH 7.5, 25°C, electron acceptor: cytochrome c Rhodotorula graminis

Organism

Organism UniProt Comment Textmining
Rhodotorula graminis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-mandelate + 2 ferricyanide
-
 Rhodotorula graminis 2-oxo-2-phenylacetate + 2 ferrocyanide + 2 H+
-
 ?
(S)-mandelate + 2,6-dichloroindophenol
-
 Rhodotorula graminis 2-oxo-2-phenylacetate + ?
-
 ?
(S)-mandelate + cytochrome c
-
 Rhodotorula graminis 2-oxo-2-phenylacetate + ?
-
 ?

Synonyms

Synonyms Comment Organism
L-mandelate dehydrogenase
-
 Rhodotorula graminis
L-MDH
-
 Rhodotorula graminis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3 6 (S)-Mandelate pH 7.5, 25°C, electron acceptor: 2,6-dichloroindophenol Rhodotorula graminis
49
-
 (S)-Mandelate pH 7.5, 25°C, electron acceptor: cytochrome c Rhodotorula graminis
109
-
 (S)-Mandelate pH 7.5, 25°C, electron acceptor: ferricyanide Rhodotorula graminis

Cofactor

Cofactor Comment Organism Structure
flavocytochrome b2 the enzyme is a flavocytochrome b2 Rhodotorula graminis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.078
-
 oxalate pH 7.5, 25°C Rhodotorula graminis
0.37
-
 hexanoate pH 7.5, 25°C Rhodotorula graminis
0.4
-
 L-lactate pH 7.5, 25°C Rhodotorula graminis
1.9
-
 L-Phenyllactate pH 7.5, 25°C Rhodotorula graminis