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Literature summary for 1.1.99.30 extracted from

  • Neumann, S.; Simon, H.
    On a non-pyridine nucleotide-dependent 2-oxoacid reductase of broad specificity from two Proteus species (1985), FEBS Lett., 167, 29-32.
No PubMed abstract available
Search for more literature for 1.1.99.30

Application

Application Comment Organism
synthesis enzyme can be used as a stereospecific biocatalyst for production of a wide range of 2-hydroxy-carboxylate compounds at preparative scale, with cofactor methyl or benzyl viologen being more stable than NAD(P)H Proteus mirabilis
synthesis enzyme can be used as a stereospecific biocatalyst for production of a wide range of 2-hydroxy-carboxylate compounds at preparative scale, with cofactor methyl or benzyl viologen being more stable than NAD(P)H Proteus vulgaris

Inhibitors

Inhibitors Comment Organism Structure
CN- 30% inhibition at 10 mM Proteus mirabilis
CN- 30% inhibition at 10 mM Proteus vulgaris
additional information no inhibition by CO Proteus mirabilis
additional information no inhibition by CO Proteus vulgaris

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Proteus mirabilis 16020
-
membrane bound Proteus vulgaris 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum dependent on Proteus mirabilis

Organism

Organism UniProt Comment Textmining
Proteus mirabilis
-
-
-
Proteus sp.
-
-
-
Proteus vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Proteus vulgaris
167fold Proteus mirabilis

Source Tissue

Source Tissue Comment Organism Textmining
additional information aerobic growth conditions Proteus mirabilis
-
additional information anaerobic growth conditions Proteus vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
600
-
 purified enzyme Proteus mirabilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-2-oxo-3-methylpentanoate + reduced benzyl viologen
-
 Proteus vulgaris 2-hydroxy-3-methylpentanoate + oxidized benzyl viologen
-
 ?
(R,S)-2-oxo-3-methylpentanoate + reduced methyl viologen
-
 Proteus vulgaris 2-hydroxy-3-methylpentanoate + oxidized methyl viologen
-
 ?
(S)-2-oxo-3-methylpentanoate + reduced benzyl viologen
-
 Proteus mirabilis 2-hydroxy-3-methylpentanoate + oxidized benzyl viologen
-
 ?
(S)-2-oxo-3-methylpentanoate + reduced benzyl viologen
-
 Proteus vulgaris 2-hydroxy-3-methylpentanoate + oxidized benzyl viologen
-
 ?
(S)-2-oxo-3-methylpentanoate + reduced methyl viologen
-
 Proteus mirabilis 2-hydroxy-3-methylpentanoate + oxidized methyl viologen
-
 ?
(S)-2-oxo-3-methylpentanoate + reduced methyl viologen
-
 Proteus vulgaris 2-hydroxy-3-methylpentanoate + oxidized methyl viologen
-
 ?
2-oxo-3,3-dimethyl-4-hydroxybutanoate + reduced benzyl viologen
-
 Proteus mirabilis 3,3-dimethyl-2,4-dihydroxybutanoate + oxidized benzyl viologen
-
 ?
2-oxo-3,3-dimethyl-4-hydroxybutanoate + reduced benzyl viologen
-
 Proteus vulgaris 3,3-dimethyl-2,4-dihydroxybutanoate + oxidized benzyl viologen
-
 ?
2-oxo-3,3-dimethyl-4-hydroxybutanoate + reduced methyl viologen
-
 Proteus mirabilis 3,3-dimethyl-2,4-dihydroxybutanoate + oxidized methyl viologen
-
 ?
2-oxo-3,3-dimethyl-4-hydroxybutanoate + reduced methyl viologen
-
 Proteus vulgaris 3,3-dimethyl-2,4-dihydroxybutanoate + oxidized methyl viologen
-
 ?
2-oxo-4-(hydroxy-methyl-phosphinyl)-butanoate + reduced benzyl viologen
-
 Proteus mirabilis 2-hydroxy-4-(hydroxymethylphosphinyl)-butanoate + oxidized benzyl viologen
-
 ?
2-oxo-4-(hydroxy-methyl-phosphinyl)-butanoate + reduced methyl viologen
-
 Proteus mirabilis 2-hydroxy-4-(hydroxymethylphosphinyl)-butanoate + oxidized methyl viologen
-
 ?
2-oxo-4-methylpentanoate + reduced benzyl viologen
-
 Proteus mirabilis 2-hydroxy-4-methylpentanoate + oxidized benzyl viologen
-
 ?
2-oxo-4-methylpentanoate + reduced methyl viologen
-
 Proteus mirabilis 2-hydroxy-4-methylpentanoate + oxidized methyl viologen
-
 ?
2-oxoadipate + reduced benzyl viologen
-
 Proteus mirabilis 2-hydroxyadipate + oxidized benzyl viologen
-
 ?
2-oxoadipate + reduced methyl viologen
-
 Proteus mirabilis 2-hydroxyadipate + oxidized methyl viologen
-
 ?
2-oxoglutarate + reduced benzyl viologen
-
 Proteus mirabilis 2-hydroxyglutarate + oxidized benzyl viologen
-
 ?
2-oxoglutarate + reduced benzyl viologen
-
 Proteus vulgaris 2-hydroxyglutarate + oxidized benzyl viologen
-
 ?
2-oxoglutarate + reduced methyl viologen
-
 Proteus mirabilis 2-hydroxyglutarate + oxidized methyl viologen
-
 ?
2-oxoglutarate + reduced methyl viologen
-
 Proteus vulgaris 2-hydroxyglutarate + oxidized methyl viologen
-
 ?
2-oxononanoate + reduced benzyl viologen
-
 Proteus mirabilis 2-hydroxynonanoate + oxidized benzyl viologen
-
 ?
2-oxononanoate + reduced methyl viologen
-
 Proteus mirabilis 2-hydroxynonanoate + oxidized methyl viologen
-
 ?
3-fluoropyruvate + reduced benzyl viologen
-
 Proteus mirabilis 3-fluoro-2-hydroxypropionate + oxidized benzyl viologen
-
 ?
3-fluoropyruvate + reduced benzyl viologen
-
 Proteus vulgaris 3-fluoro-2-hydroxypropionate + oxidized benzyl viologen
-
 ?
3-fluoropyruvate + reduced methyl viologen
-
 Proteus mirabilis 3-fluoro-2-hydroxypropionate + oxidized methyl viologen
-
 ?
3-fluoropyruvate + reduced methyl viologen
-
 Proteus vulgaris 3-fluoro-2-hydroxypropionate + oxidized methyl viologen
-
 ?
5-benzyloxyindolylpyruvate + reduced benzyl viologen
-
 Proteus mirabilis 3-(5-benzyloxyindolyl)-2-hydroxypropionate + oxidized benzyl viologen
-
 ?
5-benzyloxyindolylpyruvate + reduced methyl viologen
-
 Proteus mirabilis 3-(5-benzyloxyindolyl)-2-hydroxypropionate + oxidized methyl viologen
-
 ?
a (2R)-hydroxy-carboxylate + acceptor
-
 Proteus sp. a 2-oxo-carboxylate + reduced acceptor
-
 ?
indolylpyruvate + reduced benzyl viologen
-
 Proteus mirabilis 2-hydroxy-3-indolylpropionate + oxidized benzyl viologen
-
 ?
indolylpyruvate + reduced benzyl viologen
-
 Proteus vulgaris 2-hydroxy-3-indolylpropionate + oxidized benzyl viologen
-
 ?
indolylpyruvate + reduced methyl viologen
-
 Proteus mirabilis 2-hydroxy-3-indolylpropionate + oxidized methyl viologen
-
 ?
indolylpyruvate + reduced methyl viologen
-
 Proteus vulgaris 2-hydroxy-3-indolylpropionate + oxidized methyl viologen
-
 ?
additional information broad substrate specificity, stereochemic specificity, no activity with 3-oxoglutarate and hydroxyacetone Proteus mirabilis ?
-
 ?
additional information broad substrate specificity, stereochemic specificity, no activity with 3-oxoglutarate and hydroxyacetone Proteus vulgaris ?
-
 ?
phenylglyoxylate + reduced benzyl viologen
-
 Proteus mirabilis (R)-mandelic acid
-
 ?
phenylglyoxylate + reduced benzyl viologen
-
 Proteus vulgaris (R)-mandelic acid
-
 ?
phenylglyoxylate + reduced methyl viologen
-
 Proteus mirabilis (R)-mandelic acid
-
 ?
phenylglyoxylate + reduced methyl viologen
-
 Proteus vulgaris (R)-mandelic acid
-
 ?
phenylpyruvate + reduced benzyl viologen
-
 Proteus mirabilis 2-hydroxy-3-phenylpropionate + oxidized benzyl viologen
-
 ?
phenylpyruvate + reduced benzyl viologen
-
 Proteus vulgaris 2-hydroxy-3-phenylpropionate + oxidized benzyl viologen
-
 ?
phenylpyruvate + reduced methyl viologen
-
 Proteus mirabilis 2-hydroxy-3-phenylpropionate + oxidized methyl viologen
-
 ?
phenylpyruvate + reduced methyl viologen
-
 Proteus vulgaris 2-hydroxy-3-phenylpropionate + oxidized methyl viologen
-
 ?
pyruvate + reduced benzyl viologen
-
 Proteus mirabilis lactate + oxidized benzyl viologen
-
 ?
pyruvate + reduced benzyl viologen
-
 Proteus vulgaris lactate + oxidized benzyl viologen
-
 ?
pyruvate + reduced methyl viologen
-
 Proteus mirabilis lactate + oxidized methyl viologen
-
 ?
pyruvate + reduced methyl viologen
-
 Proteus vulgaris lactate + oxidized methyl viologen
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Proteus mirabilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
 room temperature, 30 h, stable Proteus mirabilis
22
-
 room temperature, 30 h, stable Proteus vulgaris

Cofactor

Cofactor Comment Organism Structure
additional information non-pyridine nucleotide-dependent Proteus mirabilis
additional information non-pyridine nucleotide-dependent Proteus vulgaris