General Stability | Organism |
---|---|
amphoteric detergents, such as dimethyldodecylamineoxide, lauroylsarcosine, dodecylsulfobetaine, lauroylbetaine are effective solubilizers | Gluconobacter oxydans |
dimethyldodecylamineoxide, 0.5%, stabilizes | Gluconobacter oxydans |
glycerol stabilizes | Gluconobacter oxydans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
glycerol | membrane fraction | Gluconobacter oxydans | |
19 | - |
meso-erythritol | - |
Gluconobacter oxydans | |
22 | - |
D-arabitol | - |
Gluconobacter oxydans | |
34 | - |
glycerol | solubilized enzyme | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Gluconobacter oxydans | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
maximum activity at the end of exponential growth phase, activity occurs in various Gluconobacter species, such as Gluconobacter cerinus, Gluconobacter gluconicus, Gluconobacter suboxydans and Gluconobacter albidus | - |
Gluconobacter oxydans IFO 3260 | - |
maximum activity at the end of exponential growth phase, activity occurs in various Gluconobacter species, such as Gluconobacter cerinus, Gluconobacter gluconicus, Gluconobacter suboxydans and Gluconobacter albidus | - |
Purification (Comment) | Organism |
---|---|
- |
Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
52 | - |
- |
Gluconobacter oxydans |
Storage Stability | Organism |
---|---|
-20°C, solubilized, stable for 6 months | Gluconobacter oxydans |
2-4°C, diluted enzyme solution, stable for 2 weeks | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adonitol + acceptor | 42% of activity with glycerol | Gluconobacter oxydans | ? | - |
? | |
adonitol + acceptor | 42% of activity with glycerol | Gluconobacter oxydans IFO 3260 | ? | - |
? | |
D-arabitol + acceptor | 136% of activity with glycerol oxidation | Gluconobacter oxydans | ? | - |
? | |
D-arabitol + acceptor | 136% of activity with glycerol oxidation | Gluconobacter oxydans IFO 3260 | ? | - |
? | |
D-mannitol + acceptor | 26% of activity with glycerol | Gluconobacter oxydans | D-fructose + reduced acceptor | - |
? | |
D-sorbitol + acceptor | 45% of acticvity with glycerol | Gluconobacter oxydans | L-sorbose + reduced acceptor | - |
? | |
D-sorbitol + acceptor | 45% of acticvity with glycerol | Gluconobacter oxydans IFO 3260 | L-sorbose + reduced acceptor | - |
? | |
galactitol + acceptor | i.e. dulcitol, 29% of activity with glycerol | Gluconobacter oxydans | ? | - |
? | |
glycerol + acceptor | 2,6-dichlorophenolindophenol/phenazine methosulfate act as in vitro acceptor, broad specificity, no substrates are: xylitol, D-fructose, D-glucose, D-gluconate, 2-keto-D-gluconate, ethanol and acetaldehyde | Gluconobacter oxydans | glycerone + reduced acceptor | i.e. dihydroxyacetone | ? | |
glycerol + acceptor | 2,6-dichlorophenolindophenol/phenazine methosulfate act as in vitro acceptor, broad specificity, no substrates are: xylitol, D-fructose, D-glucose, D-gluconate, 2-keto-D-gluconate, ethanol and acetaldehyde | Gluconobacter oxydans IFO 3260 | glycerone + reduced acceptor | i.e. dihydroxyacetone | ? | |
meso-erythritol + acceptor | 111% of activity with glycerol | Gluconobacter oxydans | ? | - |
? | |
meso-erythritol + acceptor | 111% of activity with glycerol | Gluconobacter oxydans IFO 3260 | ? | - |
? | |
propylene glycol + acceptor | 54% of activity with glycerol | Gluconobacter oxydans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | could be identical with non-purified EC 1.1.2.2 or D-arabitol dehydrogenase | Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Gluconobacter oxydans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10 | stable in the presence of glycerol and detergent | Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2,6-dichlorophenolindophenol | acts in vitro as acceptor together with phenazine methosulfate | Gluconobacter oxydans | |
additional information | NAD+, NADP+ or molecular oxygen do not act as acceptors | Gluconobacter oxydans | |
phenazine methosulfate | acts in vitro as acceptor together with 2,6-dichlorophenolindophenol, linked to electron-transport chain of the organism | Gluconobacter oxydans | |
pyrroloquinoline quinone | quinoprotein | Gluconobacter oxydans |