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Literature summary for 1.1.99.20 extracted from

  • Yamanaka, H.; Kawai, F.
    Inducible or constitutive polyethylene glycol dehydrogenase involved in the aerobic metabolism of polyethylene glycol (1989), J. Ferment. Bioeng., 67, 300-302.
No PubMed abstract available
Search for more literature for 1.1.99.20

Activating Compound

Activating Compound Comment Organism Structure
ethylamine 30% activation, Pseudomonas sp. strain No. 43 Pseudomonas sp.
methylamine 30% activation, Pseudomonas sp. strain No. 43 Pseudomonas sp.
phenazine methosulfate activation, accelerates dichlorophenolindophenol reduction Pseudomonas sp.
phenazine methosulfate activation, accelerates dichlorophenolindophenol reduction Flavobacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
1,4-benzoquinone at 0.02-0.05 mM, strong inhibitor; competitive to 2,6-dichlorophenolindophenol Flavobacterium sp.
1,4-benzoquinone at 0.02-0.05 mM, strong inhibitor; competitive to 2,6-dichlorophenolindophenol Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Pseudomonas sp. 16020
-
membrane
-
 Flavobacterium sp. 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 30% activation, Pseudomonas sp. strain No. 101 Pseudomonas sp.
additional information no metal ion requirement Pseudomonas sp.
additional information no metal ion requirement Flavobacterium sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
 strains No.202 to 205
-
Flavobacterium sp.
-
 synergistic mixed culture with Pseudomonas sp.
-
Pseudomonas sp.
-
 strains No.42, 43, 101, 103
-
Pseudomonas sp.
-
 synergistic mixed culture with Flavobacterium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.028
-
 strain No. 43 Pseudomonas sp.
0.035
-
 strain No. 203, crude extract Flavobacterium sp.
0.164
-
 strain No. 103 Pseudomonas sp.
1.2
-
 mixed culture Pseudomonas sp.
1.2
-
 mixed culture Flavobacterium sp.
2.68
-
 strain No. 203, purified enzyme Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
PEG-1000 + acceptor
-
 Pseudomonas sp. ?
-
 ?
PEG-1000 + acceptor
-
 Flavobacterium sp. ?
-
 ?
PEG-200 + acceptor
-
 Pseudomonas sp. ?
-
 ?
PEG-200 + acceptor
-
 Flavobacterium sp. ?
-
 ?
PEG-20000 + acceptor
-
 Pseudomonas sp. ?
-
 ?
PEG-20000 + acceptor
-
 Flavobacterium sp. ?
-
 ?
PEG-300 + acceptor
-
 Pseudomonas sp. ?
-
 ?
PEG-300 + acceptor
-
 Flavobacterium sp. ?
-
 ?
PEG-400 + acceptor
-
 Pseudomonas sp. ?
-
 ?
PEG-400 + acceptor
-
 Flavobacterium sp. ?
-
 ?
PEG-4000 + acceptor
-
 Pseudomonas sp. ?
-
 ?
PEG-4000 + acceptor
-
 Flavobacterium sp. ?
-
 ?
PEG-6000 + acceptor
-
 Pseudomonas sp. ?
-
 ?
PEG-6000 + acceptor
-
 Flavobacterium sp. ?
-
 ?
polyethylene glycol + acceptor constitutive quinoprotein enzyme, enzyme of Flavobacterium sp. No.203 inductive Flavobacterium sp. ?
-
 ?
polyethylene glycol + acceptor first and main step in aerobic PEG-metabolism Pseudomonas sp. ?
-
 ?
polyethylene glycol + acceptor first and main step in aerobic PEG-metabolism Flavobacterium sp. ?
-
 ?
primary alcohol + acceptor 2,6-dichlorophenolindophenol is the acceptor in vitro Pseudomonas sp. aldehyde + reduced acceptor
-
 ?
primary alcohol + acceptor 2,6-dichlorophenolindophenol is the acceptor in vitro Flavobacterium sp. aldehyde + reduced acceptor
-
 ?

Cofactor

Cofactor Comment Organism Structure
2,6-dichlorophenolindophenol electron acceptor Pseudomonas sp.
2,6-dichlorophenolindophenol electron acceptor Flavobacterium sp.