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Literature summary for 1.1.99.2 extracted from

  • Suzuki, T.; Uozomi, T.; Beppu, T.
    Purification and characterization of a-hydroxyglutarate dehydrogenase from Alcaligenes sp. (1985), Agric. Biol. Chem., 49, 2939-2947.
No PubMed abstract available
Search for more literature for 1.1.99.2

Crystallization (Commentary)

Crystallization (Comment) Organism
concentrated by ultrafiltration and crystallized by vapor diffusion method Alcaligenes sp.

Inhibitors

Inhibitors Comment Organism Structure
(R)-2-hydroxyglutarate
-
 Alcaligenes sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 2-oxoglutarate
-
 Alcaligenes sp.
0.14
-
 NADH
-
 Alcaligenes sp.
1.7
-
 (S)-2-hydroxyglutarate
-
 Alcaligenes sp.
5.3
-
 NAD+
-
 Alcaligenes sp.
15
-
 (R)-2-hydroxyglutarate
-
 Alcaligenes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 1 * 42000, SDS-PAGE Alcaligenes sp.
48000
-
 gel filtration Alcaligenes sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Alcaligenes sp. high levels of the enzyme is produced at the late stage of cultivation in the presence of citrate and with limited aeration ?
-
 ?

Organism

Organism UniProt Comment Textmining
Alcaligenes sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Alcaligenes sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture high levels of the enzyme is produced at the late stage of cultivation in the presence of citrate and with limited aeration Alcaligenes sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Alcaligenes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-hydroxyglutarate + NAD+ about 10% of the activity with (S)-2-hydroxyglutarate Alcaligenes sp. 2-oxoglutarate + NADH
-
 ir
(S)-2-hydroxyglutarate + NAD+ in the reverse reaction almost 100% production of the S-isomer of 2-hydroxyglutarate Alcaligenes sp. 2-oxoglutarate + NADH + H+
-
 r
2-hydroxy-n-hexanoate + NAD+ at 12.4% of the activity with 2-oxoglutarate Alcaligenes sp. 2-oxo-n-hexanoate + NADH + H+
-
 ?
2-hydroxy-n-pentanoate + NAD+ at 10.7% of the activity with 2-oxoglutarate Alcaligenes sp. 2-oxo-n-pentanoate + NADH + H+
-
 ?
2-hydroxybutanoate + NAD+ at 9.6% of the activity with 2-oxoglutarate Alcaligenes sp. 2-oxobutanoate + NADH
-
 ?
2-hydroxymalonate + NAD+ at 29.3% of the activity with 2-oxoglutarate Alcaligenes sp. oxomalonate + NADH + H+
-
 ?
additional information high levels of the enzyme is produced at the late stage of cultivation in the presence of citrate and with limited aeration Alcaligenes sp. ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 42000, SDS-PAGE Alcaligenes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 reaction with alpha-oxoglutarate and NADH Alcaligenes sp.
11.5
-
 reaction with L-2-hydroxyglutarate and NAD+ Alcaligenes sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 pH 5.0: about 45% of maximal activity, pH 8.5: 60% of maximal activity, reaction with alpha-oxoglutarate and NADH Alcaligenes sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Alcaligenes sp.
NADH
-
 Alcaligenes sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
45
-
 (R)-2-hydroxyglutarate
-
 Alcaligenes sp.