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Literature summary for 1.1.99.18 extracted from

  • Ma, S.; Preims, M.; Piumi, F.; Kappel, L.; Seiboth, B.; Record, E.; Kracher, D.; Ludwig, R.
    Molecular and catalytic properties of fungal extracellular cellobiose dehydrogenase produced in prokaryotic and eukaryotic expression systems (2017), Microb. Cell Fact., 16, 37.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pichia pastoris, Aspergillus niger, and Trichoderma reesei Thermothelomyces fergusii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.081
-
cellobiose recombinant enzyme from Pichia pastoris, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C Thermothelomyces fergusii
0.102
-
cellobiose recombinant enzyme from Aspergillus niger, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C Thermothelomyces fergusii
0.118
-
cellobiose recombinant enzyme from Trichoderma reesei, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C Thermothelomyces fergusii
0.349
-
cellobiose recombinant enzyme from Trichoderma reesei, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C Thermothelomyces fergusii
0.357
-
cellobiose recombinant enzyme from Aspergillus niger, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C Thermothelomyces fergusii
0.367
-
cellobiose recombinant enzyme from Escherichia coli, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C Thermothelomyces fergusii
0.373
-
cellobiose recombinant enzyme from Pichia pastoris, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C Thermothelomyces fergusii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
x * 53000, SDS-PAGE Thermothelomyces fergusii

Organism

Organism UniProt Comment Textmining
Thermothelomyces fergusii
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Thermothelomyces fergusii

Purification (Commentary)

Purification (Comment) Organism
IMAC, phenyl Sepharose column chromatography, and QSource column chromatography, Thermothelomyces fergusii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichlorophenolindophenol
-
Thermothelomyces fergusii cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
cellobiose + ferricytochrome c
-
Thermothelomyces fergusii cellobiono-1,5-lactone + ferrocytochrome c
-
?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Thermothelomyces fergusii

Synonyms

Synonyms Comment Organism
Cdh
-
Thermothelomyces fergusii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6
-
cellobiose recombinant enzyme from Pichia pastoris, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C Thermothelomyces fergusii
7.6
-
cellobiose recombinant enzyme from Aspergillus niger, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C Thermothelomyces fergusii
7.9
-
cellobiose recombinant enzyme from Trichoderma reesei, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C Thermothelomyces fergusii
8.1
-
cellobiose recombinant enzyme from Pichia pastoris, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C Thermothelomyces fergusii
13.3
-
cellobiose recombinant enzyme from Aspergillus niger, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C Thermothelomyces fergusii
15.2
-
cellobiose recombinant enzyme from Trichoderma reesei, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C Thermothelomyces fergusii
15.5
-
cellobiose recombinant enzyme from Escherichia coli, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C Thermothelomyces fergusii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
22
-
cellobiose recombinant enzyme from Pichia pastoris, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C Thermothelomyces fergusii
32
-
cellobiose recombinant enzyme from Pichia pastoris, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C Thermothelomyces fergusii
37
-
cellobiose recombinant enzyme from Aspergillus niger, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C Thermothelomyces fergusii
42
-
cellobiose recombinant enzyme from Escherichia coli, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C Thermothelomyces fergusii
44
-
cellobiose recombinant enzyme from Trichoderma reesei, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C Thermothelomyces fergusii
67
-
cellobiose recombinant enzyme from Trichoderma reesei, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C Thermothelomyces fergusii
74
-
cellobiose recombinant enzyme from Aspergillus niger, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C Thermothelomyces fergusii