Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Pichia pastoris, Aspergillus niger, and Trichoderma reesei | Thermothelomyces fergusii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.081 | - |
cellobiose | recombinant enzyme from Pichia pastoris, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C | Thermothelomyces fergusii | |
0.102 | - |
cellobiose | recombinant enzyme from Aspergillus niger, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C | Thermothelomyces fergusii | |
0.118 | - |
cellobiose | recombinant enzyme from Trichoderma reesei, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C | Thermothelomyces fergusii | |
0.349 | - |
cellobiose | recombinant enzyme from Trichoderma reesei, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C | Thermothelomyces fergusii | |
0.357 | - |
cellobiose | recombinant enzyme from Aspergillus niger, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C | Thermothelomyces fergusii | |
0.367 | - |
cellobiose | recombinant enzyme from Escherichia coli, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C | Thermothelomyces fergusii | |
0.373 | - |
cellobiose | recombinant enzyme from Pichia pastoris, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C | Thermothelomyces fergusii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, SDS-PAGE | Thermothelomyces fergusii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermothelomyces fergusii | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Thermothelomyces fergusii |
Purification (Comment) | Organism |
---|---|
IMAC, phenyl Sepharose column chromatography, and QSource column chromatography, | Thermothelomyces fergusii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + 2,6-dichlorophenolindophenol | - |
Thermothelomyces fergusii | cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
cellobiose + ferricytochrome c | - |
Thermothelomyces fergusii | cellobiono-1,5-lactone + ferrocytochrome c | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, SDS-PAGE | Thermothelomyces fergusii |
Synonyms | Comment | Organism |
---|---|---|
Cdh | - |
Thermothelomyces fergusii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
cellobiose | recombinant enzyme from Pichia pastoris, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C | Thermothelomyces fergusii | |
7.6 | - |
cellobiose | recombinant enzyme from Aspergillus niger, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C | Thermothelomyces fergusii | |
7.9 | - |
cellobiose | recombinant enzyme from Trichoderma reesei, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C | Thermothelomyces fergusii | |
8.1 | - |
cellobiose | recombinant enzyme from Pichia pastoris, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C | Thermothelomyces fergusii | |
13.3 | - |
cellobiose | recombinant enzyme from Aspergillus niger, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C | Thermothelomyces fergusii | |
15.2 | - |
cellobiose | recombinant enzyme from Trichoderma reesei, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C | Thermothelomyces fergusii | |
15.5 | - |
cellobiose | recombinant enzyme from Escherichia coli, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C | Thermothelomyces fergusii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
cellobiose | recombinant enzyme from Pichia pastoris, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C | Thermothelomyces fergusii | |
32 | - |
cellobiose | recombinant enzyme from Pichia pastoris, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C | Thermothelomyces fergusii | |
37 | - |
cellobiose | recombinant enzyme from Aspergillus niger, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C | Thermothelomyces fergusii | |
42 | - |
cellobiose | recombinant enzyme from Escherichia coli, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C | Thermothelomyces fergusii | |
44 | - |
cellobiose | recombinant enzyme from Trichoderma reesei, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C | Thermothelomyces fergusii | |
67 | - |
cellobiose | recombinant enzyme from Trichoderma reesei, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C | Thermothelomyces fergusii | |
74 | - |
cellobiose | recombinant enzyme from Aspergillus niger, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C | Thermothelomyces fergusii |