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Literature summary for 1.1.99.18 extracted from

  • Choi, H.; Kim, D.; Thapa, L.; Lee, S.; Kim, S.; Cho, J.; Park, C.; Kim, S.
    Production and characterization of cellobiose dehydrogenase from Phanerochaete chrysosporium KCCM 60256 and its application for an enzymatic fuel cell (2016), Korean J. Chem. Engin., 33, 3434-3441.
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris X-33 Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 cellobiose at pH 4.5 and 30°C Phanerodontia chrysosporium
0.34
-
 lactose at pH 4.5 and 30°C Phanerodontia chrysosporium
1.47
-
 D-glucose at pH 4.5 and 30°C Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 x * 100000, SDS-PAGE Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
-
-
Phanerodontia chrysosporium KCCM 60256
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ProBond resin (Ni2+) column chromatography Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichlorophenolindophenol
-
 Phanerodontia chrysosporium cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
cellobiose + 2,6-dichlorophenolindophenol
-
 Phanerodontia chrysosporium KCCM 60256 cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
D-glucose + 2,6-dichlorophenolindophenol
-
 Phanerodontia chrysosporium D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
D-glucose + 2,6-dichlorophenolindophenol
-
 Phanerodontia chrysosporium KCCM 60256 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
lactose + 2,6-dichlorophenolindophenol
-
 Phanerodontia chrysosporium lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
lactose + 2,6-dichlorophenolindophenol
-
 Phanerodontia chrysosporium KCCM 60256 lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

Subunits Comment Organism
? x * 100000, SDS-PAGE Phanerodontia chrysosporium

Synonyms

Synonyms Comment Organism
Cdh
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Phanerodontia chrysosporium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 the enzyme is highly stable at 30-60°C, with maximum activity at 30°C when incubated for 5 min. Below 30°C and above 60°C, the enzyme activity decreases, respectively Phanerodontia chrysosporium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.22
-
 D-glucose at pH 4.5 and 30°C Phanerodontia chrysosporium
6.27
-
 cellobiose at pH 4.5 and 30°C Phanerodontia chrysosporium
8.2
-
 lactose at pH 4.5 and 30°C Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Phanerodontia chrysosporium

pH Range

pH Minimum pH Maximum Comment Organism
4 6.5 the relative activity of purified enzyme is decreased when pH is over 4.5 and it is below 80% at 6.5 Phanerodontia chrysosporium