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Literature summary for 1.1.99.18 extracted from

  • Harreither, W.; Nicholls, P.; Sygmund, C.; Gorton, L.; Ludwig, R.
    Investigation of the pH-dependent electron transfer mechanism of ascomycetous class II cellobiose dehydrogenases on electrodes (2012), Langmuir, 28, 6714-6723.
    View publication on PubMed
Search for more literature for 1.1.99.18

Application

Application Comment Organism
biotechnology class II cellobiose dehydrogenases are potential candidates for glucose biosensors and biofuel cell anodes Neurospora crassa
biotechnology class II cellobiose dehydrogenases are potential candidates for glucose biosensors and biofuel cell anodes Thermothelomyces fergusii
biotechnology class II cellobiose dehydrogenases are potential candidates for glucose biosensors and biofuel cell anodes Stachybotrys bisbyi
biotechnology class II cellobiose dehydrogenases are potential candidates for glucose biosensors and biofuel cell anodes Amesia atrobrunnea
biotechnology class II cellobiose dehydrogenases are potential candidates for glucose biosensors and biofuel cell anodes Dichomera saubinetii
biotechnology class II cellobiose dehydrogenases are potential candidates for glucose biosensors and biofuel cell anodes Hypoxylon haematostroma

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 cellobiose at pH 5.0 and 30°C Amesia atrobrunnea
0.0024
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Stachybotrys bisbyi
0.003
-
 cellobiose at pH 7.5 and 30°C Thermothelomyces fergusii
0.0046
-
 cytochrome c at pH 7.5 and 30°C Thermothelomyces fergusii
0.0049
-
 cytochrome c at pH 5.0 and 30°C Stachybotrys bisbyi
0.005
-
 cellobiose at pH 5.0 and 30°C Hypoxylon haematostroma
0.0056
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Hypoxylon haematostroma
0.0057
-
 cytochrome c at pH 5.0 and 30°C Dichomera saubinetii
0.0058
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Dichomera saubinetii
0.007
-
 cellobiose at pH 6.0 and 30°C Neurospora crassa
0.007
-
 cellobiose at pH 5.0 and 30°C Stachybotrys bisbyi
0.008
-
 cytochrome c at pH 5.0 and 30°C Amesia atrobrunnea
0.0087
-
 2,6-dichlorophenol indophenol at pH 5.5 and 30°C Neurospora crassa
0.0092
-
 2,6-dichlorophenol indophenol at pH 6.0 and 30°C Amesia atrobrunnea
0.012
-
 cellobiose at pH 5.0 and 30°C Dichomera saubinetii
0.014
-
 lactose at pH 7.5 and 30°C Thermothelomyces fergusii
0.018
-
 lactose at pH 5.0 and 30°C Amesia atrobrunnea
0.0259
-
 cytochrome c at pH 5.0 and 30°C Hypoxylon haematostroma
0.028
-
 lactose at pH 6.0 and 30°C Neurospora crassa
0.032
-
 lactose at pH 5.0 and 30°C Stachybotrys bisbyi
0.048
-
 lactose at pH 5.0 and 30°C Dichomera saubinetii
0.05
-
 lactose at pH 5.0 and 30°C Hypoxylon haematostroma
0.0537
-
 cytochrome c at pH 6.0 and 30°C Neurospora crassa
0.0698
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Thermothelomyces fergusii
2.4
-
 maltose at pH 7.5 and 30°C Thermothelomyces fergusii
7.1
-
 maltose at pH 5.0 and 30°C Hypoxylon haematostroma
7.8
-
 maltose at pH 5.0 and 30°C Amesia atrobrunnea
13
-
 maltose at pH 5.0 and 30°C Dichomera saubinetii
21
-
 D-glucose at pH 7.5 and 30°C Thermothelomyces fergusii
24
-
 maltose at pH 5.0 and 30°C Stachybotrys bisbyi
31
-
 maltose at pH 6.0 and 30°C Neurospora crassa
170
-
 D-glucose at pH 6.0 and 30°C Neurospora crassa
230
-
 D-glucose at pH 5.0 and 30°C Stachybotrys bisbyi
260
-
 D-glucose at pH 5.0 and 30°C Hypoxylon haematostroma
770
-
 D-glucose at pH 5.0 and 30°C Dichomera saubinetii
1700
-
 D-glucose at pH 5.0 and 30°C Amesia atrobrunnea

Organism

Organism UniProt Comment Textmining
Amesia atrobrunnea
-
-
-
Dichomera saubinetii
-
-
-
Hypoxylon haematostroma
-
-
-
Neurospora crassa
-
-
-
Stachybotrys bisbyi
-
-
-
Thermothelomyces fergusii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + cytochrome c
-
 Neurospora crassa cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
cellobiose + cytochrome c
-
 Thermothelomyces fergusii cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
cellobiose + cytochrome c
-
 Stachybotrys bisbyi cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
cellobiose + cytochrome c
-
 Amesia atrobrunnea cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
cellobiose + cytochrome c
-
 Dichomera saubinetii cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
cellobiose + cytochrome c
-
 Hypoxylon haematostroma cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
D-glucose + 2,6-dichlorophenol indophenol
-
 Neurospora crassa D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
D-glucose + 2,6-dichlorophenol indophenol
-
 Thermothelomyces fergusii D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
D-glucose + 2,6-dichlorophenol indophenol
-
 Stachybotrys bisbyi D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
D-glucose + 2,6-dichlorophenol indophenol
-
 Amesia atrobrunnea D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
D-glucose + 2,6-dichlorophenol indophenol
-
 Dichomera saubinetii D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
D-glucose + 2,6-dichlorophenol indophenol
-
 Hypoxylon haematostroma D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Neurospora crassa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Thermothelomyces fergusii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Stachybotrys bisbyi 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Amesia atrobrunnea 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Dichomera saubinetii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Hypoxylon haematostroma 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
maltose + oxidized 2,6-dichlorophenolindophenol
-
 Neurospora crassa ? + reduced 2,6-dichlorophenolindophenol
-
 ?
maltose + oxidized 2,6-dichlorophenolindophenol
-
 Thermothelomyces fergusii ? + reduced 2,6-dichlorophenolindophenol
-
 ?
maltose + oxidized 2,6-dichlorophenolindophenol
-
 Stachybotrys bisbyi ? + reduced 2,6-dichlorophenolindophenol
-
 ?
maltose + oxidized 2,6-dichlorophenolindophenol
-
 Amesia atrobrunnea ? + reduced 2,6-dichlorophenolindophenol
-
 ?
maltose + oxidized 2,6-dichlorophenolindophenol
-
 Dichomera saubinetii ? + reduced 2,6-dichlorophenolindophenol
-
 ?
maltose + oxidized 2,6-dichlorophenolindophenol
-
 Hypoxylon haematostroma ? + reduced 2,6-dichlorophenolindophenol
-
 ?

Synonyms

Synonyms Comment Organism
Cdh
-
 Neurospora crassa
Cdh
-
 Thermothelomyces fergusii
Cdh
-
 Stachybotrys bisbyi
Cdh
-
 Amesia atrobrunnea
Cdh
-
 Dichomera saubinetii
Cdh
-
 Hypoxylon haematostroma

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 maltose at pH 5.0 and 30°C Stachybotrys bisbyi
0.3
-
 maltose at pH 6.0 and 30°C Neurospora crassa
0.5
-
 D-glucose at pH 5.0 and 30°C Hypoxylon haematostroma
1
-
 maltose at pH 5.0 and 30°C Amesia atrobrunnea
1.2
-
 maltose at pH 5.0 and 30°C Dichomera saubinetii
1.6
-
 D-glucose at pH 5.0 and 30°C Stachybotrys bisbyi
1.9
-
 D-glucose at pH 6.0 and 30°C Neurospora crassa
2.1
-
 cellobiose at pH 5.0 and 30°C Stachybotrys bisbyi
2.3
-
 lactose at pH 5.0 and 30°C Stachybotrys bisbyi
3.3
-
 cellobiose at pH 6.0 and 30°C Neurospora crassa
3.4
-
 lactose at pH 6.0 and 30°C Neurospora crassa
3.6
-
 maltose at pH 5.0 and 30°C Hypoxylon haematostroma
3.9
-
 lactose at pH 5.0 and 30°C Hypoxylon haematostroma
4
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Stachybotrys bisbyi
4.9
-
 cytochrome c at pH 5.0 and 30°C Stachybotrys bisbyi
5
-
 maltose at pH 7.5 and 30°C Thermothelomyces fergusii
7.1
-
 D-glucose at pH 5.0 and 30°C Amesia atrobrunnea
7.7
-
 cellobiose at pH 5.0 and 30°C Amesia atrobrunnea
8.2
-
 lactose at pH 5.0 and 30°C Amesia atrobrunnea
9.2
-
 lactose at pH 5.0 and 30°C Dichomera saubinetii
9.4
-
 cellobiose at pH 5.0 and 30°C Dichomera saubinetii
10
-
 D-glucose at pH 7.5 and 30°C Thermothelomyces fergusii
10.9
-
 lactose at pH 7.5 and 30°C Thermothelomyces fergusii
11.8
-
 cellobiose at pH 7.5 and 30°C Thermothelomyces fergusii
13.5
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Hypoxylon haematostroma
16.2
-
 D-glucose at pH 5.0 and 30°C Dichomera saubinetii
16.2
-
 cellobiose at pH 5.0 and 30°C Hypoxylon haematostroma
17
-
 2,6-dichlorophenol indophenol at pH 6.0 and 30°C Amesia atrobrunnea
18.1
-
 cytochrome c at pH 5.0 and 30°C Hypoxylon haematostroma
21.3
-
 cytochrome c at pH 5.0 and 30°C Amesia atrobrunnea
21.4
-
 cytochrome c at pH 7.5 and 30°C Thermothelomyces fergusii
22.6
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Dichomera saubinetii
24.8
-
 2,6-dichlorophenol indophenol at pH 5.5 and 30°C Neurospora crassa
25.2
-
 cytochrome c at pH 5.0 and 30°C Dichomera saubinetii
28.7
-
 cytochrome c at pH 6.0 and 30°C Neurospora crassa
53.8
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Thermothelomyces fergusii

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Neurospora crassa
FAD
-
 Thermothelomyces fergusii
FAD
-
 Stachybotrys bisbyi
FAD
-
 Amesia atrobrunnea
FAD
-
 Dichomera saubinetii
FAD
-
 Hypoxylon haematostroma
heme b
-
 Neurospora crassa
heme b
-
 Thermothelomyces fergusii
heme b
-
 Stachybotrys bisbyi
heme b
-
 Amesia atrobrunnea
heme b
-
 Dichomera saubinetii
heme b
-
 Hypoxylon haematostroma

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.007
-
 D-glucose at pH 5.0 and 30°C Stachybotrys bisbyi
0.009
-
 maltose at pH 5.0 and 30°C Stachybotrys bisbyi
0.01
-
 maltose at pH 6.0 and 30°C Neurospora crassa
0.011
-
 D-glucose at pH 6.0 and 30°C Neurospora crassa
0.014
-
 D-glucose at pH 5.0 and 30°C Hypoxylon haematostroma
0.021
-
 D-glucose at pH 5.0 and 30°C Dichomera saubinetii
0.048
-
 D-glucose at pH 7.5 and 30°C Thermothelomyces fergusii
0.067
-
 maltose at pH 5.0 and 30°C Hypoxylon haematostroma
0.09
-
 maltose at pH 5.0 and 30°C Dichomera saubinetii
0.128
-
 D-glucose at pH 5.0 and 30°C Amesia atrobrunnea
0.208
-
 maltose at pH 7.5 and 30°C Thermothelomyces fergusii
71
-
 lactose at pH 5.0 and 30°C Stachybotrys bisbyi
71
-
 lactose at pH 5.0 and 30°C Hypoxylon haematostroma
120
-
 lactose at pH 6.0 and 30°C Neurospora crassa
190
-
 lactose at pH 5.0 and 30°C Dichomera saubinetii
220
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Thermothelomyces fergusii
320
-
 cellobiose at pH 5.0 and 30°C Stachybotrys bisbyi
450
-
 cellobiose at pH 6.0 and 30°C Neurospora crassa
450
-
 maltose at pH 5.0 and 30°C Amesia atrobrunnea
540
-
 cytochrome c at pH 6.0 and 30°C Neurospora crassa
700
-
 cytochrome c at pH 5.0 and 30°C Hypoxylon haematostroma
760
-
 cellobiose at pH 5.0 and 30°C Dichomera saubinetii
780
-
 lactose at pH 7.5 and 30°C Thermothelomyces fergusii
850
-
 cellobiose at pH 5.0 and 30°C Hypoxylon haematostroma
1000
-
 cytochrome c at pH 5.0 and 30°C Stachybotrys bisbyi
1700
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Stachybotrys bisbyi
1800
-
 2,6-dichlorophenol indophenol at pH 6.0 and 30°C Amesia atrobrunnea
2400
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Hypoxylon haematostroma
2700
-
 cellobiose at pH 5.0 and 30°C Amesia atrobrunnea
2900
-
 2,6-dichlorophenol indophenol at pH 5.5 and 30°C Neurospora crassa
3100
-
 cytochrome c at pH 7.5 and 30°C Thermothelomyces fergusii
3300
-
 lactose at pH 5.0 and 30°C Amesia atrobrunnea
3900
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Dichomera saubinetii
4000
-
 cellobiose at pH 7.5 and 30°C Thermothelomyces fergusii
4400
-
 cytochrome c at pH 5.0 and 30°C Dichomera saubinetii