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Literature summary for 1.1.99.18 extracted from

  • Flitsch, A.; Prasetyo, E.N.; Sygmund, C.; Ludwig, R.; Nyanhongo, G.S.; Guebitz, G.M.
    Cellulose oxidation and bleaching processes based on recombinant Myriococcum thermophilum cellobiose dehydrogenase (2013), Enzyme Microb. Technol., 52, 60-67.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris X-33 cells Thermothelomyces myriococcoides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
cellobiose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
0.085
-
cellotetraose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
0.09
-
lactose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
0.71
-
maltotetraose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
6.75
-
maltose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
387
-
D-glucose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides

Organism

Organism UniProt Comment Textmining
Thermothelomyces myriococcoides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and phenyl Sepharose column chromatography Thermothelomyces myriococcoides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
culture supernatant, in 100 mM sodium acetate buffer, pH 5.0, at 30°C Thermothelomyces myriococcoides
3.1
-
after 15.5fold purification, in 100 mM sodium acetate buffer, pH 5.0, at 30°C Thermothelomyces myriococcoides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichlorophenol indophenol
-
Thermothelomyces myriococcoides cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
cellotetraose + 2,6-dichlorophenol indophenol
-
Thermothelomyces myriococcoides cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
D-glucose + 2,6-dichlorophenol indophenol weak substrate Thermothelomyces myriococcoides D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
lactose + 2,6-dichlorophenol indophenol
-
Thermothelomyces myriococcoides 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
-
Thermothelomyces myriococcoides 4-O-(alpha-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
maltotetraose + 2,6-dichlorophenol indophenol
-
Thermothelomyces myriococcoides ? + reduced 2,6-dichlorophenol indophenol
-
?
maltotriose + 2,6-dichlorophenol indophenol
-
Thermothelomyces myriococcoides ? + reduced 2,6-dichlorophenol indophenol
-
?

Synonyms

Synonyms Comment Organism
Cdh
-
Thermothelomyces myriococcoides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.315
-
maltose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
0.48
-
maltotetraose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
2.7
-
cellotetraose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
4.11
-
cellobiose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
4.68
-
lactose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
4.8
-
D-glucose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.012
-
D-glucose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
0.047
-
maltose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
0.676
-
maltotetraose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
32.12
-
cellotetraose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
52
-
lactose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
117.1
-
cellobiose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides