Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain X-33 | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
cellobiose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
0.043 | - |
cellopentaose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
0.045 | - |
cellotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
0.061 | - |
cellotetraose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
0.089 | - |
lactose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
0.196 | - |
cellopentaose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
0.204 | - |
cellotetraose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
0.23 | - |
cellotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
0.29 | - |
lactose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
0.81 | - |
xylotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
0.9 | - |
cellobiose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.2 | - |
maltotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.31 | - |
xylobiose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
3 | - |
xylotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
3.44 | - |
maltose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
3.6 | - |
xylobiose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
4.5 | - |
maltotetraose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
8.4 | - |
Mannopentaose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
17.2 | - |
maltose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
34.3 | - |
maltotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
35.7 | - |
maltotetraose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
39.2 | - |
Mannopentaose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
550 | - |
D-glucose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1545 | - |
D-xylose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
2770 | - |
D-xylose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
3600 | - |
D-galactose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
3700 | - |
D-glucose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
10000 | - |
D-mannose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
13300 | - |
D-mannose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
x * 85000, isoform CDH IIA, SDS-PAGE | Neurospora crassa |
85800 | - |
x * 85800, isoform CDH IIB, SDS-PAGE | Neurospora crassa |
110000 | 130000 | gel filtration | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + 1,4-benzoquinone | - |
Neurospora crassa | cellobiono-1,5-lactone + reduced 1,4-benzoquinone | - |
? | |
cellobiose + 2,6-dichloroindophenol | - |
Neurospora crassa | cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
cellobiose + cytochrome c | - |
Neurospora crassa | cellobiono-1,5-lactone + reduced cytochrome c | - |
? | |
cellopentaose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
cellotetraose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
cellotriose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
cellulose + ferrocenium hexafluorophosphate | - |
Neurospora crassa | cellobiono-1,5-lactone + ferricenium hexafluorophosphate | - |
? | |
D-galactose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
D-glucose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
D-mannose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
D-xylose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
lactose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
maltose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
maltotetraose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
maltotriose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
mannopentaose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
additional information | isoform CDH IIA exhibits 4 to 5times higher apparent catalytic constants (kcat and Km values) than isoform CDH IIB for most tested carbohydrates | Neurospora crassa | ? | - |
? | |
xylobiose + 1,4-benzoquinone | - |
Neurospora crassa | 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced 1,4-benzoquinone | - |
? | |
xylotriose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 85000, isoform CDH IIA, SDS-PAGE | Neurospora crassa |
? | x * 85800, isoform CDH IIB, SDS-PAGE | Neurospora crassa |
Synonyms | Comment | Organism |
---|---|---|
CDH IIA | isoform | Neurospora crassa |
CDH IIB | isoform | Neurospora crassa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
maltose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1 | - |
maltotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.4 | - |
maltotetraose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.5 | - |
D-galactose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.5 | - |
D-xylose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.8 | - |
maltotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.9 | - |
D-xylose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
2.2 | - |
maltotetraose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
2.3 | - |
xylotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
3.5 | - |
maltose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
3.6 | - |
D-galactose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
4.3 | - |
D-mannose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
4.7 | - |
D-mannose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
5.8 | - |
xylobiose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
6 | - |
xylotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
7.4 | - |
Mannopentaose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
8.1 | - |
D-glucose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
10.2 | - |
cellopentaose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
11.4 | - |
cellobiose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
11.6 | - |
cellotetraose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
11.8 | - |
lactose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
12.4 | - |
cellotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
19.8 | - |
Mannopentaose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
45.4 | - |
xylobiose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
45.8 | - |
cellobiose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
46 | - |
cellotetraose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
46.6 | - |
cellopentaose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
49.3 | - |
lactose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
49.7 | - |
cellotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
55 | - |
D-glucose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
using 2,6-dichlorophenolindol as electron donor | Neurospora crassa |
7 | - |
using 1,4-benzoquinone as electron donor | Neurospora crassa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.7 | 8 | more than 50% relative activity from pH 3.8 to 6.5 using 1,4-benzoquinone as electron acceptor | Neurospora crassa |
3.8 | 6.5 | more than 50% relative activity from pH 3.8 to 6.5 using 2,6-dichlorophenolindol as electron acceptor | Neurospora crassa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Neurospora crassa | isoform CDH IIB, isoelectric focusing | - |
4.9 |
Neurospora crassa | isoform CDH IIA, isoelectric focusing | - |
5.1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
D-mannose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
0.0004 | - |
D-mannose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
0.0005 | - |
D-xylose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
0.0012 | - |
D-xylose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
0.014 | - |
D-glucose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
0.015 | - |
D-glucose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
0.023 | - |
maltose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
0.053 | - |
maltotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
0.061 | - |
maltotetraose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
0.506 | - |
Mannopentaose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
0.8 | - |
maltotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
0.88 | - |
Mannopentaose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.02 | - |
maltose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
2 | - |
xylotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
2.74 | - |
maltotetraose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
2.8 | - |
xylotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
4.45 | - |
xylobiose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
12.8 | - |
xylobiose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
133 | - |
lactose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
170 | - |
lactose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
189 | - |
cellotetraose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
216 | - |
cellotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
226 | - |
cellotetraose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
238 | - |
cellopentaose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
238 | - |
cellopentaose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
278 | - |
cellotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
508 | - |
cellobiose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
527 | - |
cellobiose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa |