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Literature summary for 1.1.99.18 extracted from

  • Morpeth, F.F.
    Some properties of cellobiose oxidase from the white-rot fungus Sporotrichum pulverulentum (1985), Biochem. J., 228, 557-564.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.99.18

Inhibitors

Inhibitors Comment Organism Structure
cellobiose strong substrate inhibition at concentrations higher than 0.015 mM Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 2,6-dichlorophenolindophenol in presence of 0.24 mM O2 Phanerodontia chrysosporium
0.017
-
 2,6-dichlorophenolindophenol in absence of O2 Phanerodontia chrysosporium
0.042
-
 cellobiose
-
 Phanerodontia chrysosporium
0.58
-
 lactose
-
 Phanerodontia chrysosporium
3
-
 benzyl viologen
-
 Phanerodontia chrysosporium
6.6
-
 maltose
-
 Phanerodontia chrysosporium

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Phanerodontia chrysosporium
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74400
-
 sedimentation equilibrium centrifugation Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
 white-rot fungus
-
Phanerodontia chrysosporium
-
 identical with Sporotrichum pulverulentum, 3 isoenzymes
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content: 11.9% Phanerodontia chrysosporium

Purification (Commentary)

Purification (Comment) Organism
70fold purification Phanerodontia chrysosporium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Phanerodontia chrysosporium

Storage Stability

Storage Stability Organism
4°C, 50 mM potassium phosphate buffer, pH 6, several months, stable, slowly loss of activity Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + O2 immediate reduction of enzyme Phanerodontia chrysosporium ?
-
 ?
cellobiose + benzyl viologen
-
 Phanerodontia chrysosporium cellobiono-1,5-lactone + reduced benzyl viologen
-
 r
cellobiose + O2
-
 Phanerodontia chrysosporium cellobiono-1,5-lactone + H2O2 superoxide anion as primary reduced oxygen product, H2O2 is possibly formed by dismutation of superoxide anion ?
cellulose + O2 immediate reduction of enzyme Phanerodontia chrysosporium ?
-
 ?
chitin + O2 immediate reduction of enzyme Phanerodontia chrysosporium ?
-
 ?
lactose + O2
-
 Phanerodontia chrysosporium 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + H2O2
-
 ?
maltose + O2
-
 Phanerodontia chrysosporium 4-O-beta-glucosyl-glucono-1,5-lactone + H2O2
-
 ?
additional information substrates: disaccharides, some insoluble polysaccharides, but no monosaccharides, oxygen as electron acceptor, artificial electron acceptors: 2,6-dichlorophenolindophenol, potassium ferricyanide, benzyl viologen, not with: FMN, FAD, riboflavin Phanerodontia chrysosporium ?
-
 ?
xylan + O2 immediate reduction of enzyme Phanerodontia chrysosporium ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Phanerodontia chrysosporium

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 11 pH 2: below large loss of activity, pH 11: above large loss of activity Phanerodontia chrysosporium

Cofactor

Cofactor Comment Organism Structure
cytochrome b as prosthetic group Phanerodontia chrysosporium
FAD as prosthetic group Phanerodontia chrysosporium
flavin flavoprotein, FAD as flavin component Phanerodontia chrysosporium