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Literature summary for 1.1.99.18 extracted from

  • Bao, W.; Usha, S.N.; Renganathan, V.
    Purification and characterization of cellobiose dehydrogenase, a novel extracellular hemoflavoenzyme from the white-rot fungus Phanerochoaete chrysosporium (1993), Arch. Biochem. Biophys., 300, 705-713.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
cytochrome c
-
Phanerodontia chrysosporium
0.0036
-
2,6-dichlorophenol-indophenol
-
Phanerodontia chrysosporium
0.0052
-
ferricyanide
-
Phanerodontia chrysosporium
0.012
-
3,5-di-tert-butyl-1,2-benzoquinone
-
Phanerodontia chrysosporium
0.025
-
cellobiose
-
Phanerodontia chrysosporium
0.055
-
cellopentaose
-
Phanerodontia chrysosporium
0.061
-
cellotetraose
-
Phanerodontia chrysosporium
0.062
-
cellotriose
-
Phanerodontia chrysosporium
0.15
-
Mn3+-malonate
-
Phanerodontia chrysosporium
0.63
-
lactose
-
Phanerodontia chrysosporium

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Phanerodontia chrysosporium
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
gel filtration Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
white-rot fungus
-
Phanerodontia chrysosporium OGC 101
-
white-rot fungus
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 9.4% carbohydrate content Phanerodontia chrysosporium

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-Sephadex, Phenyl-Sepharose, Sephacryl S-200, Mono Q Phanerodontia chrysosporium

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Phanerodontia chrysosporium
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.3
-
-
Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + acceptor Cytochrome c, 2,6-dichlorophenolindophenol, ferricyanide, 3,5-di-tert-butyl-1,2-benzoquinone, Mn3+-malonate can serve as electron acceptors. O2 is a poor acceptor in the absence of artificial acceptors and is reduced to H2O2. Molecular oxygen acts as two-electron acceptor, superoxide is produced through an iron mediated pathway Phanerodontia chrysosporium cellobiono-1,5-lactone + reduced acceptor
-
?
cellobiose + acceptor Cytochrome c, 2,6-dichlorophenolindophenol, ferricyanide, 3,5-di-tert-butyl-1,2-benzoquinone, Mn3+-malonate can serve as electron acceptors. O2 is a poor acceptor in the absence of artificial acceptors and is reduced to H2O2. Molecular oxygen acts as two-electron acceptor, superoxide is produced through an iron mediated pathway Phanerodontia chrysosporium OGC 101 cellobiono-1,5-lactone + reduced acceptor
-
?
cellobiose + ferricyanide
-
Phanerodontia chrysosporium cellobiono-1,5-lactone + ferrocyanide
-
r
cellobiose + Mn(III)-malonate
-
Phanerodontia chrysosporium cellobiono-1,5-lactone + reduced Mn(III)-malonate
-
r
cellobiose + Mn3+-malonate
-
Phanerodontia chrysosporium cellobiono-1,5-lactone + reduced Mn3+-malonate
-
r
cellopentaose + acceptor
-
Phanerodontia chrysosporium cellopentaono-1,5-lactone + reduced acceptor
-
?
cellopentaose + acceptor
-
Phanerodontia chrysosporium OGC 101 cellopentaono-1,5-lactone + reduced acceptor
-
?
cellotetraose + acceptor
-
Phanerodontia chrysosporium cellotetrono-1,5-lactone + reduced acceptor
-
?
cellotetraose + acceptor
-
Phanerodontia chrysosporium OGC 101 cellotetrono-1,5-lactone + reduced acceptor
-
?
cellotriose + acceptor
-
Phanerodontia chrysosporium cellotriono-1,5-lactone + reduced acceptor
-
?
cellotriose + acceptor
-
Phanerodontia chrysosporium OGC 101 cellotriono-1,5-lactone + reduced acceptor
-
?
lactose + 3,5-di-tert-butyl-1,2-benzoquinone
-
Phanerodontia chrysosporium 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + ?
-
?
lactose + acceptor
-
Phanerodontia chrysosporium 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
?
lactose + acceptor
-
Phanerodontia chrysosporium OGC 101 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
?

Subunits

Subunits Comment Organism
monomer 1 * 90000, SDS-PAGE Phanerodontia chrysosporium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 20% activity lost after 12 h at 50°C and pH 6.1, 80% activity lost after 1 h at 60°C and pH 6.1, all activity lost after 10 min at 70°C and pH 6.1 Phanerodontia chrysosporium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14
-
Mn(III)-malonate
-
Phanerodontia chrysosporium
20.5
-
cytochrome c
-
Phanerodontia chrysosporium
20.7
-
cellotetraose
-
Phanerodontia chrysosporium
21.5
-
cellopentaose
-
Phanerodontia chrysosporium
22
-
ferricyanide
-
Phanerodontia chrysosporium
24
-
cellobiose
-
Phanerodontia chrysosporium
24
-
cellotriose
-
Phanerodontia chrysosporium
27
-
3,5-di-tert-butyl-1,2-benzoquinone
-
Phanerodontia chrysosporium
28.8
-
lactose
-
Phanerodontia chrysosporium
33
-
2,6-dichlorophenol-indophenol
-
Phanerodontia chrysosporium

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10.5 complete inactivation at pH 2 within 2 h Phanerodontia chrysosporium

Cofactor

Cofactor Comment Organism Structure
FAD hemoflavoenzyme, heme b/FAD/enzyme ratio of 1/1/1 Phanerodontia chrysosporium
heme b hemoflavoenzyme, heme b/FAD/enzyme ratio of 1/1/1 Phanerodontia chrysosporium