General Stability | Organism |
---|---|
stable to repeated freezing and thawing | Athelia rolfsii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0416 | - |
cellobiose | - |
Athelia rolfsii | |
0.0625 | - |
cellotriose | - |
Athelia rolfsii | |
0.0666 | - |
cellotetraose | - |
Athelia rolfsii | |
0.083 | - |
cellopentaose | - |
Athelia rolfsii | |
0.1 | - |
cellohexaose | - |
Athelia rolfsii | |
0.22 | - |
2,6-dichlorophenolindophenol | - |
Athelia rolfsii | |
0.384 | - |
cytochrome c | - |
Athelia rolfsii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | bound to mycelium from which it is released into the extracellular medium | Athelia rolfsii | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63100 | - |
gel permeation | Athelia rolfsii |
64500 | - |
1 * 64500, SDS-PAGE | Athelia rolfsii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Athelia rolfsii | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 8.9% carbohydrate | Athelia rolfsii |
Purification (Comment) | Organism |
---|---|
- |
Athelia rolfsii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Athelia rolfsii | - |
culture medium | bound to mycelium from which it is released into the extracellular medium | Athelia rolfsii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26.9 | - |
- |
Athelia rolfsii |
Storage Stability | Organism |
---|---|
-15°C, pH 4.5 | Athelia rolfsii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + acceptor | artificial electron acceptors: 2,6-dichlorophenolindophenol, 1,4-benzoquinone, 2,6-dimethyl-1,4-benzoquinone, 2,6-dimethoxy-1,4-benzoquinone, 3,5-di-tert-butyl-1,2-benzoquinone, methylene blue and green, meldola blue, 2,2'-azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid), cytochrome c, K3Fe(CN)6 | Athelia rolfsii | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
cellobiose + cytochrome c | - |
Athelia rolfsii | cellobiono-1,5-lactone + reduced cytochrome c | - |
r | |
cellohexaose + acceptor | - |
Athelia rolfsii | cellohexaono-1,5-lactone + reduced acceptor | - |
? | |
cellopentaose + acceptor | - |
Athelia rolfsii | cellopentaono-1,5-lactone + reduced acceptor | - |
? | |
cellotetraose + acceptor | - |
Athelia rolfsii | cellotetrono-1,5-lactone + reduced acceptor | - |
? | |
cellotriose + acceptor | - |
Athelia rolfsii | cellotriono-1,5-lactone + reduced acceptor | - |
? | |
lactose + acceptor | - |
Athelia rolfsii | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 64500, SDS-PAGE | Athelia rolfsii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Athelia rolfsii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
10 min, 86% loss of activity | Athelia rolfsii |
100 | - |
2 min, complete loss of activity | Athelia rolfsii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | 6.4 | - |
Athelia rolfsii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | most stable at | Athelia rolfsii |