Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-oxyquinoline | 80% residual activity at 1 mM | Trichoderma harzianum | |
Co2+ | 88% residual activity at 1 mM | Trichoderma harzianum | |
Cu2+ | complete inhibition at 1 mM | Trichoderma harzianum | |
DW 1750000 | 91% residual activity at 1 mM | Trichoderma harzianum | |
Fe3+ | 73% residual activity at 1 mM | Trichoderma harzianum | |
glyoxylate | 79% residual activity at 1 mM | Trichoderma harzianum | |
Hg2+ | 2% residual activity at 1 mM | Trichoderma harzianum | |
hydrazine | 77% residual activity at 1 mM | Trichoderma harzianum | |
hydroxylamine | 83% residual activity at 1 mM | Trichoderma harzianum | |
iodoacetic acid | 63% residual activity at 1 mM | Trichoderma harzianum | |
Mn2+ | complete inhibition at 1 mM | Trichoderma harzianum | |
additional information | not inhibited by semicarbazide, 4-chloromercuribenzoic acid and EDTA | Trichoderma harzianum | |
N-ethylmaleimide | 56% residual activity at 1 mM | Trichoderma harzianum | |
Ni2+ | complete inhibition at 1 mM | Trichoderma harzianum | |
o-phenanthroline | 88% residual activity at 1 mM | Trichoderma harzianum | |
Sodium azide | 85% residual activity at 1 mM | Trichoderma harzianum | |
Zn2+ | 76% residual activity at 1 mM | Trichoderma harzianum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
Nitro blue tetrazolium | at pH 7.0 and 30°C | Trichoderma harzianum | |
0.94 | - |
glycolate | at pH 7.0 and 30°C | Trichoderma harzianum | |
3.6 | - |
L-lactate | at pH 7.0 and 30°C | Trichoderma harzianum | |
49.5 | - |
dihydroxyacetone | at pH 7.0 and 30°C | Trichoderma harzianum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
1 * 107000 + 1 * 81000 + 1 * 53000 + 1 * 38000, SDS-PAGE | Trichoderma harzianum |
53000 | - |
1 * 107000 + 1 * 81000 + 1 * 53000 + 1 * 38000, SDS-PAGE | Trichoderma harzianum |
81000 | - |
1 * 107000 + 1 * 81000 + 1 * 53000 + 1 * 38000, SDS-PAGE | Trichoderma harzianum |
107000 | - |
1 * 107000 + 1 * 81000 + 1 * 53000 + 1 * 38000, SDS-PAGE | Trichoderma harzianum |
280000 | - |
gel filtration | Trichoderma harzianum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Trichoderma harzianum | the enzyme does not require NAD+ and NADP+ as an electron carrier, and primary alcohols, dihydric alcohols and aliphatic aldehydes are not oxidized. Ethanol, 1-propanol, 2-propanol, 1-butanol, ethylene glycol, propylene glycol, 1,3-propanediol, glycerol, glyoxylate, glyoxal, methylglyoxal, acetaldehyde, propylaldehyde, L-malic acid, L-kactic acid, D-malic acid and L-tartaric acid are inert | ? | - |
? | |
additional information | Trichoderma harzianum AIU 353 | the enzyme does not require NAD+ and NADP+ as an electron carrier, and primary alcohols, dihydric alcohols and aliphatic aldehydes are not oxidized. Ethanol, 1-propanol, 2-propanol, 1-butanol, ethylene glycol, propylene glycol, 1,3-propanediol, glycerol, glyoxylate, glyoxal, methylglyoxal, acetaldehyde, propylaldehyde, L-malic acid, L-kactic acid, D-malic acid and L-tartaric acid are inert | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma harzianum | - |
- |
- |
Trichoderma harzianum AIU 353 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Toyopearl column chromatography, Q-Toyopearl column chromatography, and Toyopearl, GigaCap Q-Toyopearl column chromatography, hydroxyapatite column chromatography, and gel filtration | Trichoderma harzianum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00165 | - |
crude cell extract, at pH 7.0 and 30°C | Trichoderma harzianum |
0.727 | - |
after 440fold purification, at pH 7.0 and 30°C | Trichoderma harzianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone + nitro blue tetrazolium | 162% activity compared to glycolate | Trichoderma harzianum | ? + reduced nitro blue tetrazolium | - |
? | |
dihydroxyacetone + nitro blue tetrazolium | 162% activity compared to glycolate | Trichoderma harzianum AIU 353 | ? + reduced nitro blue tetrazolium | - |
? | |
DL-glyceraldehyde + nitro blue tetrazolium | 78% activity compared to glycolate | Trichoderma harzianum | ? + reduced nitro blue tetrazolium | - |
? | |
DL-glyceraldehyde + nitro blue tetrazolium | 78% activity compared to glycolate | Trichoderma harzianum AIU 353 | ? + reduced nitro blue tetrazolium | - |
? | |
DL-lactate + nitro blue tetrazolium | 235% activity compared to glycolate | Trichoderma harzianum | ? + reduced nitro blue tetrazolium | - |
? | |
DL-lactate + nitro blue tetrazolium | 235% activity compared to glycolate | Trichoderma harzianum AIU 353 | ? + reduced nitro blue tetrazolium | - |
? | |
glycolaldehyde + nitro blue tetrazolium | 38% activity compared to glycolate | Trichoderma harzianum | ? + reduced nitro blue tetrazolium | - |
? | |
glycolate + 2,6-dichlorophenolindophenol | - |
Trichoderma harzianum | glyoxylate + reduced 2,6-dichlorophenolindophenol | - |
? | |
glycolate + 2,6-dichlorophenolindophenol | - |
Trichoderma harzianum AIU 353 | glyoxylate + reduced 2,6-dichlorophenolindophenol | - |
? | |
glycolate + nitro blue tetrazolium | 100% activity | Trichoderma harzianum | glyoxylate + reduced nitro blue tetrazolium | - |
? | |
L-lactate + nitro blue tetrazolium | 232% activity compared to glycolate | Trichoderma harzianum | ? + reduced nitro blue tetrazolium | - |
? | |
methyl glycolate + nitro blue tetrazolium | 82% activity compared to glycolate | Trichoderma harzianum | methyl glyoxylate + reduced nitro blue tetrazolium | - |
? | |
additional information | the enzyme does not require NAD+ and NADP+ as an electron carrier, and primary alcohols, dihydric alcohols and aliphatic aldehydes are not oxidized. Ethanol, 1-propanol, 2-propanol, 1-butanol, ethylene glycol, propylene glycol, 1,3-propanediol, glycerol, glyoxylate, glyoxal, methylglyoxal, acetaldehyde, propylaldehyde, L-malic acid, L-kactic acid, D-malic acid and L-tartaric acid are inert | Trichoderma harzianum | ? | - |
? | |
additional information | the enzyme does not require NAD+ and NADP+ as an electron carrier, and primary alcohols, dihydric alcohols and aliphatic aldehydes are not oxidized. Ethanol, 1-propanol, 2-propanol, 1-butanol, ethylene glycol, propylene glycol, 1,3-propanediol, glycerol, glyoxylate, glyoxal, methylglyoxal, acetaldehyde, propylaldehyde, L-malic acid, L-kactic acid, D-malic acid and L-tartaric acid are inert | Trichoderma harzianum AIU 353 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 1 * 107000 + 1 * 81000 + 1 * 53000 + 1 * 38000, SDS-PAGE | Trichoderma harzianum |
Synonyms | Comment | Organism |
---|---|---|
GADH | - |
Trichoderma harzianum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Trichoderma harzianum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | when the enzyme is incubated with 0.1 M potassium phosphate buffer, pH 6.0, at 2060°C for 30 min, approximately 90% of the enzyme activity remain below 20°C, but the activity completely disappears at 40°C | Trichoderma harzianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Trichoderma harzianum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 6.5 | more than 80% of enzyme activity remains in the pH range between 5.0 and 6.5 for 20 min at 20°C | Trichoderma harzianum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trichoderma harzianum | isoelectric focusing | - |
5.1 |