Cloned (Comment) | Organism |
---|---|
gene glcD, expression of N-terminally Strep-tagged enzyme in Escherichia coli strain BL21 | Synechocystis sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of GlcD-deficient mutants, co-mutation of other enzymes of the alternative bacterial glycerate pathway, progressive segregation, genotyping and metabolic analysis, growth phenotypes under normal and carbon limited conditions, overview | Synechocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycolate + NAD+ | Synechocystis sp. | the enzyme is involved in the bacterial 2-phosphoglycolate pathway, overview | glyoxylate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
strain PCC 6803, gene glcD | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally Strep-tagged enzyme from Escherichia coli strain BL21 by streptavidin-tactin affinity chromatography | Synechocystis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
determination of internal glycolate concentrations | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycolate + NAD+ | - |
Synechocystis sp. | glyoxylate + NADH | - |
? | |
glycolate + NAD+ | the enzyme is involved in the bacterial 2-phosphoglycolate pathway, overview | Synechocystis sp. | glyoxylate + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlcD | - |
Synechocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Synechocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Synechocystis sp. |