Literature summary for 1.1.98.6 extracted from

Olcott, M.C.; Andersson, J.; Sjoeberg, B.M.
Localization and characterization of two nucleotide-binding sites on the anaerobic ribonucleotide reductase from bacteriophage T4 (1998), J. Biol. Chem., 273, 24853-24860.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Tequatrovirus T4

Protein Variants

Protein Variants	Comment	Organism
C290S	mutation in conserved residua, less than 10% of wild-type activity	Tequatrovirus T4

Organism

Organism	UniProt	Comment	Textmining
Tequatrovirus T4	P07071	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme displays two nucleotide-binding sites. One site exhibits half-maximal saturation at approximately 5 mM 8-azidoadenosine 5'-triphosphate, whereas the other site requires 45 microM. The higher affinity site corresponds to residues 289-291 and the other site to the region to residues 147-160. Photoinsertion of 8-azidoadenosine 5'-triphosphate into the site corresponding to residues 147-160 is almost completely abolished when 100 mM dATP, dGTP, or dTTP is included in the photolabeling reaction mixture, whereas 100 mM ATP, GTP, CTP, or dCTP have virtually no effect	Tequatrovirus T4	?	-	?

Synonyms

Synonyms	Comment	Organism
nrdD	-	Tequatrovirus T4

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Release 2023.1 (January 2023)