Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | strong stimulation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bathophenanthroline | 0.02 mM, 97% inhibition | Escherichia coli | |
Co2+ | 0.02 mM, 75% inhibition | Escherichia coli | |
desferal | 0.02 mM, 96% inhibition | Escherichia coli | |
EDTA | 0.1 mM, 96% inhibition | Escherichia coli | |
ferrozin | 0.1 mM, 72% inhibition | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
2 * 77000, SDS-PAGE | Escherichia coli |
145000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
260 | - |
pH 8.5, 22°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside 5'-triphosphate + formate | - |
Escherichia coli | 2'-deoxyribonucleoside 5'-triphosphate + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 77000, SDS-PAGE | Escherichia coli |
More | occurence of a degradation product named beta2 of 74000 Da | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is the actual ribonucleoside triphosphate reductase in the oxygen-sensitive ribonucleoside triphosphate reductase system. The reaction requires an auxiliary protein dA1 of 29000 Da, S-adenosylmethionine, NADPH (with NADH as a less active substitute), dithinthreitol, and magnesium ions, and is strongly stimulated by ATP | Escherichia coli |