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Literature summary for 1.1.5.9 extracted from
- Efficient expression, purification, and characterization of a novel FAD-dependent glucose dehydrogenase from Aspergillus terreus in Pichia pastoris (2014), J. Microbiol. Biotechnol., 24, 1516-1524.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris strain GS115 | Aspergillus terreus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 89.7 | - |
D-glucose | at pH 7.5 and 55°C | Aspergillus terreus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus terreus | Q0CDD9 | - |
- |
| Aspergillus terreus NIH2624 | Q0CDD9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isopropanol precipitation and nickel affinity column chromatography | Aspergillus terreus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha,alpha-trehalose + 2,6-dichlorophenolindophenol | 0.5% activity compared to D-glucose | Aspergillus terreus | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-galactose + 2,6-dichlorophenolindophenol | 2.2% activity compared to D-glucose | Aspergillus terreus | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-galactose + 2,6-dichlorophenolindophenol | 2.2% activity compared to D-glucose | Aspergillus terreus NIH2624 | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-glucose + 2,6-dichlorophenolindophenol | - |
Aspergillus terreus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-glucose + 2,6-dichlorophenolindophenol | - |
Aspergillus terreus NIH2624 | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-lactose + 2,6-dichlorophenolindophenol | 0.6% activity compared to D-glucose | Aspergillus terreus | D-lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-lactose + 2,6-dichlorophenolindophenol | 0.6% activity compared to D-glucose | Aspergillus terreus NIH2624 | D-lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-xylose + 2,6-dichlorophenolindophenol | 7.4% activity compared to D-glucose | Aspergillus terreus | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-xylose + 2,6-dichlorophenolindophenol | 7.4% activity compared to D-glucose | Aspergillus terreus NIH2624 | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| L-arabinose + 2,6-dichlorophenolindophenol | 1.5% activity compared to D-glucose | Aspergillus terreus | L-arabinono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| maltose + 2,6-dichlorophenolindophenol | 17.7% activity compared to D-glucose | Aspergillus terreus | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| additional information | no activity with D-fructose, S-sorbitol, and D-sucrose | Aspergillus terreus | ? | - |
? | |
| additional information | no activity with D-fructose, S-sorbitol, and D-sucrose | Aspergillus terreus NIH2624 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 83000-150000, SDS-PAGE | Aspergillus terreus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAD-dependent glucose dehydrogenase | - |
Aspergillus terreus |
| FAD-GDH | - |
Aspergillus terreus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Aspergillus terreus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 65 | about 70% activity at 30°C, about 80% activity at 35°C, about 90% activity at 45°C, about 95% activity at 50°C, 100% activity at 55°C, about 98% activity at 60°C, about 85% activity at 65°C | Aspergillus terreus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
the enzyme displays very high thermal stability with a half-life of 82 min at 60°C | Aspergillus terreus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Aspergillus terreus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | more than 70% activity between pH 6.0 and 8.5 | Aspergillus terreus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | the enzyme remains stable after 20 min at pH 3.0-9.0 | Aspergillus terreus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Aspergillus terreus | |
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