Application | Comment | Organism |
---|---|---|
analysis | possible use of the enzyme as amperometric glucose biosensor | Komagataella pastoris |
analysis | possible use of the enzyme as amperometric glucose biosensor | Colletotrichum gloeosporioides |
analysis | possible use of the enzyme as amperometric glucose biosensor | Aspergillus sp. |
Cloned (Comment) | Organism |
---|---|
recombinant expression in Pichia pastoris | Colletotrichum gloeosporioides |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the FAD-dependent glucose dehydrogenase is evaluated electrochemically connected to an osmium redox polymer [Os(4,4'-dimethyl-2,2'-bipyridine)2 (PVI)10Cl]+ on graphite electrode for possible use in glucose-based biosensors and biofuel cells, method and sensitivity, overview | Komagataella pastoris |
additional information | the FAD-dependent glucose dehydrogenase is evaluated electrochemically connected to an osmium redox polymer [Os(4,4'-dimethyl-2,2'-bipyridine)2 (PVI)10Cl]+ on graphite electrode for possible use in glucose-based biosensors and biofuel cells, method and sensitivity, overview | Colletotrichum gloeosporioides |
additional information | the FAD-dependent glucose dehydrogenase is evaluated electrochemically connected to an osmium redox polymer [Os(4,4'-dimethyl-2,2'-bipyridine)2 (PVI)10Cl]+ on graphite electrode for possible use in glucose-based biosensors and biofuel cells, method and sensitivity, overview | Aspergillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
x * 67000, deglycosylated recombinant enzyme, SDS-PAGE | Aspergillus sp. |
88000 | 131000 | - |
Komagataella pastoris |
95000 | 135000 | recombinant enzyme | Colletotrichum gloeosporioides |
97000 | - |
deglycosylated enzyme, gel filtration | Aspergillus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + a quinone | Komagataella pastoris | - |
D-glucono-1,5-lactone + a quinol | - |
? | |
D-glucose + a quinone | Colletotrichum gloeosporioides | - |
D-glucono-1,5-lactone + a quinol | - |
? | |
D-glucose + a quinone | Aspergillus sp. | - |
D-glucono-1,5-lactone + a quinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus sp. | - |
- |
- |
Colletotrichum gloeosporioides | - |
- |
- |
Komagataella pastoris | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Komagataella pastoris |
glycoprotein | - |
Colletotrichum gloeosporioides |
glycoprotein | - |
Aspergillus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Aspergillus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
795 | - |
pH and temperature not specified in the publication | Aspergillus sp. |
836 | - |
pH and temperature not specified in the publication | Komagataella pastoris |
878 | - |
pH and temperature not specified in the publication | Colletotrichum gloeosporioides |
Storage Stability | Organism |
---|---|
GcGDH/Os-polymer modified electrode under storage conditions of 50 mM phosphate buffer, pH 7.4, 4 °C, injection of 0.05 ml of a 5 mM glucose solution into the electrochemical cell for 6 days, the biosensor keeps more than 90% of its initial activity after the first day | Colletotrichum gloeosporioides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + a quinone | - |
Colletotrichum gloeosporioides | 2-deoxy-D-glucono-1,5-lactone + a quinol | - |
? | |
cellobiose + a quinone | low activity | Colletotrichum gloeosporioides | ? + a quinol | - |
? | |
D-glucose + a quinone | - |
Komagataella pastoris | D-glucono-1,5-lactone + a quinol | - |
? | |
D-glucose + a quinone | - |
Colletotrichum gloeosporioides | D-glucono-1,5-lactone + a quinol | - |
? | |
D-glucose + a quinone | - |
Aspergillus sp. | D-glucono-1,5-lactone + a quinol | - |
? | |
D-xylose + ferricenium ion | - |
Colletotrichum gloeosporioides | D-xylono-1,5-lactone + ferrocenium ion | - |
? | |
glutamine + a quinone | low activity | Colletotrichum gloeosporioides | ? + a quinol | - |
? | |
additional information | substrate specificity, overview. Lactose, gluconic acid, mannose, mannitol, sorbitol, galactose, sucrose, maltose, arabinose, xylitol, rhamnose, fucose, trehalose, and fructose are poor or no substrates | Colletotrichum gloeosporioides | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000, deglycosylated recombinant enzyme, SDS-PAGE | Aspergillus sp. |
Synonyms | Comment | Organism |
---|---|---|
FAD-dependent glucose dehydrogenase | - |
Komagataella pastoris |
FAD-dependent glucose dehydrogenase | - |
Colletotrichum gloeosporioides |
FAD-dependent glucose dehydrogenase | - |
Aspergillus sp. |
FADGDH | - |
Komagataella pastoris |
FADGDH | - |
Colletotrichum gloeosporioides |
FADGDH | - |
Aspergillus sp. |
flavin adenine dinucleotide-dependent glucose dehydrogenase | - |
Komagataella pastoris |
flavin adenine dinucleotide-dependent glucose dehydrogenase | - |
Colletotrichum gloeosporioides |
flavin adenine dinucleotide-dependent glucose dehydrogenase | - |
Aspergillus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dependent on | Komagataella pastoris | |
FAD | dependent on | Colletotrichum gloeosporioides | |
FAD | dependent on | Aspergillus sp. |