Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.5.9 extracted from

  • Sode, K.; Tsugawa, W.; Yamazaki, T.; Watanabe, M.; Ogasawara, N.; Tanaka, M.
    A novel thermostable glucose dehydrogenase varying temperature properties by altering its quaternary structures (1996), Enzyme Microb. Technol., 19, 82-85.
No PubMed abstract available

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Burkholderia cepacia 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
after preincubation at temperatures below 45°C: x * 67000 (alpha-subunit) x * 43000 (beta subunit), SDS-PAGE Burkholderia cepacia
67000
-
after preincubation at 70°C for 30 min: x * 67000 (alpha-subunit), SDS-PAGE Burkholderia cepacia
67000
-
after preincubation at temperatures below 45°C: x * 67000 (alpha-subunit) x * 43000 (beta subunit), SDS-PAGE Burkholderia cepacia
85000
-
after preincubation at 70°C for 30 min, native PAGE Burkholderia cepacia
350000
-
native PAGE Burkholderia cepacia
380000
-
gel filtration chromatography Burkholderia cepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
bacterial strain SM4 is used, isolated from a soil sample near a Japanese hot spring
-

Purification (Commentary)

Purification (Comment) Organism
-
Burkholderia cepacia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6
-
at 75°C after preincubation of the enzyme at 70°C for 30 min Burkholderia cepacia
15
-
at 70°C Burkholderia cepacia
45
-
at 45°C Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + 2,6-dichlorophenolindophenol
-
Burkholderia cepacia D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

Subunits Comment Organism
heteromer after preincubation at temperatures below 45°C: x * 67000 (alpha-subunit) x * 43000 (beta subunit), SDS-PAGE Burkholderia cepacia
homooligomer after preincubation at 70°C for 30 min: x * 67000 (alpha-subunit), SDS-PAGE Burkholderia cepacia

Synonyms

Synonyms Comment Organism
thermostable GDH
-
Burkholderia cepacia
thermostable glucose dehydrogenase
-
Burkholderia cepacia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
enzyme activity shows a second peak at 70°C with 30% of maximal activity Burkholderia cepacia
75
-
after preincubating the enzyme at 70°C for 30 min Burkholderia cepacia

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 85 20% of maximal activity at 20°C and 10% of maximal activity at 85°C, maximal activity is at 45°C with a second activity peak at 70°C (30% of maximal activity) Burkholderia cepacia
40 85 after preincubation of the enzyme at 70°C for 30 min: 15% of maximal activity at 40°C and 50% of maximal activity at 85°C, maximal activity at 75°C Burkholderia cepacia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5 70 enzyme is stable at temperatures between 5-37°C for 30 min, after incubating enzyme at 45°C for 30 min 40% of enzyme activity remains, at 70°C for 30 min 10% of enzyme activity remains Burkholderia cepacia
5 75 after preincubation at 70°C for 30 min the enzyme is stable at temperatures between 5-50°C for 30 min, after incubating enzyme at 70°C for 30 min 70% of activity remains Burkholderia cepacia