Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Acetobacter sp. | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
x * 78000 + x * 55000 + x * 18000, SDS-PAGE | Acetobacter sp. |
55000 | - |
x * 78000 + x * 55000 + x * 18000, SDS-PAGE | Acetobacter sp. |
78000 | - |
x * 78000 + x * 55000 + x * 18000, SDS-PAGE | Acetobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacter sp. | - |
isolated in Thailand | - |
Acetobacter sp. SKU 14 | - |
isolated in Thailand | - |
Purification (Comment) | Organism |
---|---|
native enzyme 130fold from membranes of glycerol-grown cells by two different steps of anion exchange chromatography, solubilization with 0.1% Triton X-100 or Tween 20, copurification with a cytochrome c | Acetobacter sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:glycerol-grown cell | - |
Acetobacter sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
293 | - |
purified native enzyme | Acetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + ferricyanide | about 40% of the activity with n-butanol | Acetobacter sp. | acetaldehyde + ferrocyanide | - |
? | |
iso-propanol + ferricyanide | about 10% of the activity with n-butanol | Acetobacter sp. | propan-2-one + ferrocyanide | - |
? | |
n-butanol + ferricyanide | - |
Acetobacter sp. | n-butanal + ferrocyanide | - |
? | |
n-pentanol + ferricyanide | about 45% of the activity with n-butanol | Acetobacter sp. | n-pentanal + ferrocyanide | - |
? | |
n-propanol + ferricyanide | about 95% of the activity with n-butanol | Acetobacter sp. | n-propanal + ferrocyanide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000 + x * 55000 + x * 18000, SDS-PAGE | Acetobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
formaldehyde-oxidizing enzyme | - |
Acetobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Acetobacter sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | 25°C: about 75% of maximal activity, 50°C: about 60% of maximal activity | Acetobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
10 min, stable | Acetobacter sp. |
45 | - |
10 min, about 50% loss of activity | Acetobacter sp. |
50 | - |
10 min, 95% loss of activity | Acetobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Acetobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | stable at 6°C, overnight | Acetobacter sp. |
10 | - |
at 6°C, overnight, about 75% inactivation | Acetobacter sp. |
11 | - |
at 6°C, overnight, complete inactivation | Acetobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the two subunits of 78000 Da and 55000 Da contain cytochrome c | Acetobacter sp. | |
pyrroloquinoline quinone | - |
Acetobacter sp. |