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Literature summary for 1.1.5.5 extracted from

  • Shinagawa, E.; Toyama, H.; Matsushita, K.; Tuitemwong, P.; Theeragool, G.; Adachi, O.
    A novel type of formaldehyde-oxidizing enzyme from the membrane of Acetobacter sp. SKU 14 (2006), Biosci. Biotechnol. Biochem., 70, 850-857.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Acetobacter sp. 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
x * 78000 + x * 55000 + x * 18000, SDS-PAGE Acetobacter sp.
55000
-
x * 78000 + x * 55000 + x * 18000, SDS-PAGE Acetobacter sp.
78000
-
x * 78000 + x * 55000 + x * 18000, SDS-PAGE Acetobacter sp.

Organism

Organism UniProt Comment Textmining
Acetobacter sp.
-
isolated in Thailand
-
Acetobacter sp. SKU 14
-
isolated in Thailand
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 130fold from membranes of glycerol-grown cells by two different steps of anion exchange chromatography, solubilization with 0.1% Triton X-100 or Tween 20, copurification with a cytochrome c Acetobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:glycerol-grown cell
-
Acetobacter sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
293
-
purified native enzyme Acetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + ferricyanide about 40% of the activity with n-butanol Acetobacter sp. acetaldehyde + ferrocyanide
-
?
iso-propanol + ferricyanide about 10% of the activity with n-butanol Acetobacter sp. propan-2-one + ferrocyanide
-
?
n-butanol + ferricyanide
-
Acetobacter sp. n-butanal + ferrocyanide
-
?
n-pentanol + ferricyanide about 45% of the activity with n-butanol Acetobacter sp. n-pentanal + ferrocyanide
-
?
n-propanol + ferricyanide about 95% of the activity with n-butanol Acetobacter sp. n-propanal + ferrocyanide
-
?

Subunits

Subunits Comment Organism
? x * 78000 + x * 55000 + x * 18000, SDS-PAGE Acetobacter sp.

Synonyms

Synonyms Comment Organism
formaldehyde-oxidizing enzyme
-
Acetobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Acetobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 25°C: about 75% of maximal activity, 50°C: about 60% of maximal activity Acetobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
10 min, stable Acetobacter sp.
45
-
10 min, about 50% loss of activity Acetobacter sp.
50
-
10 min, 95% loss of activity Acetobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
Acetobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 stable at 6°C, overnight Acetobacter sp.
10
-
at 6°C, overnight, about 75% inactivation Acetobacter sp.
11
-
at 6°C, overnight, complete inactivation Acetobacter sp.

Cofactor

Cofactor Comment Organism Structure
heme the two subunits of 78000 Da and 55000 Da contain cytochrome c Acetobacter sp.
pyrroloquinoline quinone
-
Acetobacter sp.