Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Borate | exposure of the enzyme to borate buffer of pH 10.0 results in activation of the enzyme | Aspergillus japonicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 25% inhibition at 0.01 mM | Aspergillus japonicus | |
8-hydroxyquinoline | 7.9% inhibition at 1 mM | Aspergillus japonicus | |
diethyldithiocarbamate | 40.1% inhibition at 1 mM | Aspergillus japonicus | |
dimercapto-1-propanol | 56.3% inhibition at 0.01 mM | Aspergillus japonicus | |
dithiothreitol | 26.5% inhibition at 0.01 mM | Aspergillus japonicus | |
ethyl xanthate | 27.1% inhibition at 0.1 mM | Aspergillus japonicus | |
L-ascorbic acid | 31.2% inhibition at 0.1 mM | Aspergillus japonicus | |
L-cysteine | 12.2% inhibition at 0.1 mM | Aspergillus japonicus | |
reduced glutathione | 40.8% inhibition at 0.1 mM | Aspergillus japonicus | |
Salicylaldoxime | 11.2% inhibition at 1 mM | Aspergillus japonicus | |
sodium sulfite | 46.2% inhibition at 0.1 mM | Aspergillus japonicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | contains 2 atoms of copper per mol of enzyme | Aspergillus japonicus | |
Mg2+ | activates | Aspergillus japonicus | |
Mn2+ | activates | Aspergillus japonicus | |
additional information | the enzyme does not contain molybdenum and manganese | Aspergillus japonicus | |
Ni2+ | activates | Aspergillus japonicus | |
Zn2+ | contains 0.76 atoms of zinc per mol of enzyme. Zn2+ is the most effective cation with activation being 2.3fold at a concentration of 1.0 mM | Aspergillus japonicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
400000 | - |
sedimentation equilibrium method | Aspergillus japonicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + O2 | Aspergillus japonicus | - |
glyceraldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus japonicus | - |
- |
- |
Aspergillus japonicus AT 008 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sephadex A-25 column chromatography, hydroxylapatite column chromatography, and Sephadex G-200 gel filtration | Aspergillus japonicus |
Source Tissue | Comment | Organism | Textmining |
---|
Storage Stability | Organism |
---|---|
5°C, activated enzyme at pH 8.0-10.0, 1 week, no loss of activity | Aspergillus japonicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-butanediol + O2 | - |
Aspergillus japonicus | ? | - |
? | |
1,3-butanediol + O2 | - |
Aspergillus japonicus AT 008 | ? | - |
? | |
1,3-propanediol + O2 | - |
Aspergillus japonicus | ? | - |
? | |
1,4-butanediol + O2 | - |
Aspergillus japonicus | ? | - |
? | |
1,4-butanediol + O2 | - |
Aspergillus japonicus AT 008 | ? | - |
? | |
D-fructose + O2 | slight oxidation activity | Aspergillus japonicus | ? | - |
? | |
D-fructose + O2 | slight oxidation activity | Aspergillus japonicus AT 008 | ? | - |
? | |
D-galactose + O2 | slight oxidation activity | Aspergillus japonicus | ? | - |
? | |
D-galactose + O2 | slight oxidation activity | Aspergillus japonicus AT 008 | ? | - |
? | |
dihydroxyacetone + O2 | 58.8% activity compared to glycerol | Aspergillus japonicus | 3-hydroxy-2-oxopropanal + H2O2 | - |
? | |
glycerol + O2 | - |
Aspergillus japonicus | glyceraldehyde + H2O2 | - |
? | |
additional information | no activity with glycerol 3-phosphate, methanol, ethanol, and polyvinyl alcohol | Aspergillus japonicus | ? | - |
? | |
additional information | no activity with glycerol 3-phosphate, methanol, ethanol, and polyvinyl alcohol | Aspergillus japonicus AT 008 | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 50 | the enzyme is stable up to 45°C and loses about 40% of activity at 50°C in 50 mM TES buffer, pH 7.0 | Aspergillus japonicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Aspergillus japonicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
protoheme IX | the enzyme contains 0.94 mol of heme per mol of enzyme | Aspergillus japonicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
dimercapto-1-propanol | at pH 7.0 and 37°C | Aspergillus japonicus |