Crystallization (Comment) | Organism |
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crystal structures of the GAOV Cl2-Tyr272, and F2-Tyr272 incorporated variants at 1.48, 1.23, and 1.80 A resolution, respectively. Only one halogen atom remains in the cofactor. The structurally defined catalytic center with genetic unnatural tyrosine substitution is in the radical containing form as in the wild-type, i.e., Cu(II)-(Cl-Tyr radical-Cys) or Cu(II)-(F-Tyr radical-Cys). Thus an C-F/C-Cl bond cleavage is mediated by a mononuclear copper center | Fusarium graminearum |
Protein Variants | Comment | Organism |
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additional information | genetic incorporation of 3,5-dichlorotyrosine (Cl2-Tyr) and 3,5-difluorotyrosine (F2-Tyr) to replace Tyr272 in the GAOV variant (i.e. A3.E7) optimized for expression through directed evolution. The proteins with an unnatural tyrosine residue are catalytically competent | Fusarium graminearum |
Organism | UniProt | Comment | Textmining |
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Fusarium graminearum | P0CS93 | - |
- |