KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
L-gulono-1,4-lactone | - |
Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Gallus gallus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Gallus gallus |
400000 | - |
gel filtraton | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-gulono-1,4-lactone + O2 | Gallus gallus | the enzyme has a configurational specificity for the hydroxyl group at C(2) | L-xylo-hex-3-ulonolactone + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2 | product isomerizes spontaneously to L-ascorbate | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.43 | - |
L-ascorbate synthesis | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-altrono-1,4-lactone + O2 | 16% as active as L-gulono-1,4-lactone | Gallus gallus | ? | - |
? | |
D-mannono-1,4-lactone + O2 | 64% as active as L-gulono-1,4-lactone | Gallus gallus | ? | - |
? | |
L-galactono-1,4-lactone + O2 | 90% as active as L-gulono-1,4-lactone | Gallus gallus | L-ascorbic acid + H2O2 | - |
? | |
L-gulono-1,4-lactone + O2 | the enzyme has a configurational specificity for the hydroxyl group at C-2 | Gallus gallus | L-xylo-hex-3-ulonolactone + H2O2 | product isomerizes spontaneously to L-ascorbate | ? | |
L-gulono-1,4-lactone + O2 | the enzyme has a configurational specificity for the hydroxyl group at C(2) | Gallus gallus | L-xylo-hex-3-ulonolactone + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 50000, SDS-PAGE | Gallus gallus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
in phosphate-citrate buffer | Gallus gallus |
8 | - |
in Tris-citrate buffer | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol FAD per protein monomer | Gallus gallus | |
FAD | 8alpha-(N1-histidyl)riboflavin is the cofactor structure determined by high-voltage paper electrophoresis | Gallus gallus |