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Literature summary for 1.1.3.7 extracted from

  • Michalski, C.; Mohagheghi, H.; Nimtz, M.; Pasteels, J.; Ober, D.
    Salicyl alcohol oxidase of the chemical defense secretion of two chrysomelid leaf beetles: Molecular and functional characterization of two new members of the GMC oxidoreductase gene family (2008), J. Biol. Chem., 283, 19219-19228.
    View publication on PubMed
Search for more literature for 1.1.3.7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Chrysomela tremula
expression in Escherichia coli Chrysomela populi
expression of the constructs encoding CpSAO in Escherichia coli BL21(DE3) results in the formation of insoluble inclusion bodies. The expression construct is coexpressed with the plasmid pREP4-groESL, encoding chaperonine proteins of the Hsp60 family (GroEL and GroES). Coexpression at 15°C for 16 h results in the formation of soluble recombinant SAO Chrysomela populi
expression of the constructs encoding CtSAO in Escherichia coli BL21(DE3) results in the formation of insoluble inclusion bodies. The expression construct is coexpressed with the plasmid pREP4-groESL, encoding chaperonine proteins of the Hsp60 family (GroEL and GroES). Coexpression at 15°C for 16 h results in the formation of soluble recombinant SAO Chrysomela tremula

Inhibitors

Inhibitors Comment Organism Structure
2-hydroxybenzyl alcohol substrate inhibition at concentrations of about 250 mM Chrysomela populi
2-hydroxybenzyl alcohol substrate inhibition at concentrations of about 250 mM Chrysomela tremula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
28
-
 2-hydroxybenzyl alcohol pH 6.0 Chrysomela populi
40
-
 2-hydroxybenzyl alcohol pH 6.0 Chrysomela tremula

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Chrysomela tremula
-
-
extracellular
-
 Chrysomela populi
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 apparent mass of about 80 kDa determined by SDS-PAGE, molecular mass of 67.7 kDa deduced from sequence Chrysomela populi
66700
-
 x * 66700, CpSAP without signal peptide, calculated from sequence Chrysomela populi
67100
-
 x * 67100, CtSAO without signal peptide, calculated from sequence Chrysomela tremula
67700
-
 deduced from sequence Chrysomela tremula
67700
-
 deduced from sequence Chrysomela populi
80000
-
 apparent mass of about 80 kDa determined by SDS-PAGE Chrysomela tremula
80000
-
 apparent mass of about 80 kDa determined by SDS-PAGE Chrysomela populi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-hydroxybenzyl alcohol + O2 Chrysomela tremula essential for the activation of the plant derived precursor salicin 2-hydroxybenzaldehyde + H2O2
-
 ?
2-hydroxybenzyl alcohol + O2 Chrysomela populi essential for the activation of the plant derived precursor salicin 2-hydroxybenzaldehyde + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Chrysomela populi B4F335
-
-
Chrysomela tremula B4F334
-
-
Chrysomela tremula B4F334 encoded by sao gene
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Chrysomela tremula
glycoprotein presence of hybrid type structures (GlcNAc Man5-6 GlcNAc2) Chrysomela populi
glycoprotein N-glycosylation predicted by data obtained from microsequencing Chrysomela tremula
glycoprotein N-glycosylation predicted by data obtained from microsequencing Chrysomela populi

Purification (Commentary)

Purification (Comment) Organism
-
 Chrysomela tremula
-
 Chrysomela populi
gel filtration Chrysomela tremula
gel filtration Chrysomela populi

Reaction

Reaction Comment Organism Reaction ID
an aromatic primary alcohol + O2 = an aromatic aldehyde + H2O2 belongs to the GMC oxidoreductase family (GMC: glucose-methanol-choline) Chrysomela tremula
a
an aromatic primary alcohol + O2 = an aromatic aldehyde + H2O2 belongs to the GMC oxidoreductase family (GMC: glucose-methanol-choline) Chrysomela populi
a

Source Tissue

Source Tissue Comment Organism Textmining
adult
-
 Chrysomela tremula
-
adult
-
 Chrysomela populi
-
exocrine glandular secretion from larva Chrysomela tremula
-
exocrine glandular secretion from larva Chrysomela populi
-
larva
-
 Chrysomela tremula
-
larva
-
 Chrysomela populi
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 cloning and microsequencing of peptides, substrate specificity of recombinant and native enzyme, phylogeny and comparison of sequence similarities Chrysomela populi
additional information
-
 cloning and microsequencing of peptides, substrate specificity of recombinant in comparison with native enzyme, phylogeny and comparison of sequence similarities Chrysomela tremula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxybenzyl alcohol + O2
-
 Chrysomela tremula 2-hydroxybenzaldehyde + H2O2
-
 ?
2-hydroxybenzyl alcohol + O2
-
 Chrysomela populi 2-hydroxybenzaldehyde + H2O2
-
 ?
2-hydroxybenzyl alcohol + O2 i.e. salicyl alcohol Chrysomela tremula 2-hydroxybenzaldehyde + H2O2
-
 ?
2-hydroxybenzyl alcohol + O2 i.e. salicyl alcohol Chrysomela populi 2-hydroxybenzaldehyde + H2O2
-
 ?
2-hydroxybenzyl alcohol + O2 essential for the activation of the plant derived precursor salicin Chrysomela tremula 2-hydroxybenzaldehyde + H2O2
-
 ?
2-hydroxybenzyl alcohol + O2 essential for the activation of the plant derived precursor salicin Chrysomela populi 2-hydroxybenzaldehyde + H2O2
-
 ?
2-methoxybenzyl alcohol + O2 recombinant enzyme shows 2.3% of the activity with 2-hydroxybenzyl alcohol Chrysomela tremula 2-methoxybenzylaldehyde + H2O
-
 ?
2-methoxybenzyl alcohol + O2 recombinant enzyme shows 3.2% of the activity with 2-hydroxybenzyl alcohol, native enzyme shows 14% of the activity with 2-hydroxybenzyl alcohol Chrysomela populi 2-methoxybenzylaldehyde + H2O
-
 ?
2-methylbenzyl alcohol + O2 recombinant enzyme shows 19.1% of the activity with 2-hydroxybenzyl alcohol, native enzyme shows 21% of the activity with 2-hydroxybenzyl alcohol Chrysomela populi 2-methylbenzylaldehyde + H2O
-
 ?
2-methylbenzyl alcohol + O2 recombinant enzyme shows 21.9% of the activity with 2-hydroxybenzyl alcohol Chrysomela tremula 2-methylbenzylaldehyde + H2O
-
 ?
2-phenylethyl alcohol + O2 recombinant enzyme shows 1.2% of the activity with 2-hydroxybenzyl alcohol Chrysomela tremula 2-phenylacetaldehyde + H2O
-
 ?
2-phenylethyl alcohol + O2 recombinant enzyme shows 3.8% of the activity with 2-hydroxybenzyl alcohol, native enzyme shows 21% of the activity with 2-hydroxybenzyl alcohol Chrysomela populi 2-phenylacetaldehyde + H2O
-
 ?
3-hydroxybenzyl alcohol + O2 recombinant enzyme shows 13.1% of the activity with 2-hydroxybenzyl alcohol, native enzyme shows less than 10% of the activity with 2-hydroxybenzyl alcohol Chrysomela populi 3-hydroxybenzaldehyde + H2O2
-
 ?
3-hydroxybenzyl alcohol + O2 recombinant enzyme shows 16.2% of the activity with 2-hydroxybenzyl alcohol Chrysomela tremula 3-hydroxybenzaldehyde + H2O2
-
 ?
3-methoxybenzyl alcohol + O2 recombinant enzyme shows 1% of the activity with 2-hydroxybenzyl alcohol Chrysomela tremula 3-methoxybenzylaldehyde + H2O2
-
 ?
4-hydroxybenzyl alcohol + O2 recombinant enzyme shows 2.7% of the activity with 2-hydroxybenzyl alcohol Chrysomela tremula 4-hydroxybenzaldehyde + H2O2
-
 ?
benzyl alcohol + O2 recombinant enzyme shows 12.2% of the activity with 2-hydroxybenzyl alcohol, native enzyme shows 23% of the activity with 2-hydroxybenzyl alcohol Chrysomela populi benzaldehyde + H2O2
-
 ?
benzyl alcohol + O2 recombinant enzyme shows 23.2% of the activity with 2-hydroxybenzyl alcohol Chrysomela tremula benzaldehyde + H2O2
-
 ?
additional information belongs to the GMC oxidoreductase family (GMC: glucose-methanol-choline) Chrysomela tremula ?
-
 ?
additional information belongs to the GMC oxidoreductase family (GMC: glucose-methanol-choline) Chrysomela populi ?
-
 ?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Chrysomela tremula
? x * 80000, SDS-PAGE Chrysomela populi
? x * 66700, CpSAP without signal peptide, calculated from sequence Chrysomela populi
? x * 67100, CtSAO without signal peptide, calculated from sequence Chrysomela tremula

Synonyms

Synonyms Comment Organism
CpSAO
-
 Chrysomela populi
CtSAO
-
 Chrysomela tremula
salicyl alcohol oxidase
-
 Chrysomela tremula
salicyl alcohol oxidase
-
 Chrysomela populi

pI Value

Organism Comment pI Value Maximum pI Value
Chrysomela populi calculated from sequence
-
 6.1